Customer Education

Webinar: Decreasing miRNA Sequencing Bias Using a Single Adapter and Circularization Approach

Duration: 60 minutes

Presenter: Sergio Barberan-Soler, Ph.D., Director of Sequencing Technologies, SomaGenics, Inc.

 What Does It Cover?

The ability to accurately quantify all microRNAs (miRNAs) in a sample is not only important for understanding miRNA biology, but for the development of new biomarkers and therapeutic targets. SomaGenics has developed the RealSeq®-AC library preparation kit – a new method for preparing miRNA sequencing libraries that involves ligating the miRNAs with a single adapter and circularizing the ligation products. When compared to other methods, the RealSeq®-AC kit provides greatly reduced miRNA sequencing bias and allows the identification of the largest variety of miRNAs in biological samples. This reduced bias also allows robust quantification of miRNAs present in samples across a wide range of RNA input levels.

 What Will You Learn?

  • Small RNA sequencing library preparation
  • Small RNA sequencing techniques and considerations
  • miRNA-seq bias implications
  • miRNA-seq bias reduction and techniques

 Who Should View?

  • Researchers working with small RNA transcriptomes
  • RNA-seq core facility experts and end-users
  • Small RNA/miRNA sequencing experts and end-users
  • Researchers interested in small RNA/miRNA biomarker discovery
Speaker Bio
Sergio Barberan-Soler, Ph.D.
Director of Sequencing Technologies
SomaGenics, Inc.
As the Director of Sequencing Technologies at SomaGenics, Inc., Dr. Barberan-Soler oversees technology development and innovative products to facilitate non-coding small RNA analysis by Next Generation Sequencing. Since joining SomaGenics in 2013, his work has contributed to the development of the most accurate library preparation technology to profile miRNAs from total RNA samples (RealSeq®-AC). Dr. Barberan-Soler is involved in numerous product development efforts to allow for more accurate quantification of RNA from diverse samples types. Prior to joining SomaGenics, he was a postdoctoral fellow at the CRG in Barcelona and previously received his PhD in Molecular, Cell and Developmental Biology from the University of California, Santa Cruz.