Customer Education

Seminar: Proximity Ligation Assay: Sensitive Protein Detection for Oncology Research

Seminar Title: Proximity Ligation Assay: Sensitive Protein Detection for Oncology Research

Date: Friday, November 3, 2017

Time: 10:00 AM – 11:30 AM
Check-In: 9:45 AM
Seminar: 10:00 AM – 11:00 AM
Q&A: 11:00 AM – 11:30 AM

Refreshments will be provided by MilliporeSigma for attendees.

Location: UW-Madison
WIMR II
Conference Room 6471



 Abstract

Understanding biochemical mechanisms, such as signaling pathways is critical for oncology research. These pathways are often evaluated through studying protein-protein interactions and post translational modifications (PTMs). Duolink technology, based on the Proximity Ligation Assay (PLA) is a powerful investigative tool for the detection and visualization of individual proteins, protein-protein interactions and post-translational modifications in fixed samples. The 1000X signal amplification allows for single molecule sensitivity and detection at endogenous levels using regular fluorescent or optical microscopy techniques. Duolink provides additional information to current biomarker expression profiling assays due to its ability to detect and measure functional protein complexes. The activation status of a given signaling pathway, such as receptor tyrosine kinases can be assessed in established cell lines as well as patient biopsies, and xenographs. With circulating or secreted biomarkers Duolink’s dual antibody targeting feature allows for increased specificity (benign versus malignant), sensitivity and novel biomarker identification. The ability to evaluate and quantify these proteins within a native state allows researchers to better understand the complex mechanisms that both develop and are a result of tumorigenic conditions.

Speaker Bio
Seth Hanson, MS
Academic Research Technology Specialist
Seth is a Research Technology Specialist for MilliporeSigma, covering key academic accounts in the Midwest. Seth earned his Master of Science degree in Molecular Biology from the University of Wisconsin - La Crosse. His prior research experiences include both academic and industry, with extensive work in preclinical drug development for Alzheimer’s disease and schizophrenia.



For more information, please contact technicalworkshops@sial.com or:

Kyle Pape
MilliporeSigma Account Manager
Phone: 414-519-7508
Email: kyle.pape@sial.com