Glycobiology - Carbohydrate Enzymes and Proteins

Explore our complex carbohydrate proteins, enzyme products and kits for investigating oligosaccharides, polysaccharides, and numerous additional aspects of your carbohydrate workflow.

 


Complex Carbohydrates
Enzyme Index
  Agarase
  Alginate Lyase
  α-Amylase
  β-Amylase
  Amyloglucosidase
  Cellulase
  Chitinase
  Chitosanase
  Chondroitinases
  Dextranase
  Driselase
  α-Glucosidase
  Hemicellulase
  Heparinases
  Hyaluronidase
  Inulinase
  Isoamylase
  Lysozyme
  Lyticase
  Pectinase
  Pectolyase
  Pectinesterase
  Pullulanase
  Xylanase
Carbohydrate Index
  Agarose
  Alginic Acid
  Amylose
  Amylopectin
  Cellulose
  Chitin
  Chitosan
  Chondroitin Sulfates
  Dextran
  β-Glucans
  Glycogen
  Hemicellulose
  Heparan Sulfate
  Heparin
  Hyaluronic Acid
  Inulin
  Lichenen
  Pectins
  Peptidoglycans
  Pullulan
  Starch
  Xylan
Kits and Reagents for Glycoprotein Analysis

Glycoprotein Analysis Manual


Agarase and Agarose

Agarose
Agarose is the principal neutral gelling component of agar extracted from algae. Agarose is a complex range of polysaccharide chains composed of alternating α-(1-3)-D-galactosyl-β-(1-4)-anhydro-L-galactosyl units.1

Agarase
EC# 3.2.1.81
Synonym: agarose 3-glycanohydrolase

Agarase catalyzes the hydrolysis of 1,3-b-D-galactosidic linkages in agarose, giving the tetramer as the predominant product. 1

from Pseudomonas atlantica
Product Number A6306 (lyophilized powder, ≥5,000 units/mg protein (Lowry)

References
1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/81.html)

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Alginate Lyase and Alginic Acid

Composed of blocks of β-D-(1-4) mannuronic acid homopolymeric regions (MMMM…), α-L-(1-4)guluronic acid (GGGG…) homopolymeric regions, and alternating copolymer regions of β-D-(1-4) mannuronic acid - α-L-(1-4)-guluronic acid (GMGMGM…). Bacterial alginic acid can be acetlylated at the 2 or 3 positions on mannuronic acid.

Alginate Lyase
EC# 4.2.2.3
Synonyms: poly(β-D-manuronate) lyase

Alginate lyase cleaves at the β-(1-4)-D-mannuronic bonds residues to yield oligosaccharides with 4-deoxy-α-L-erythro-hex-4-enopyranuronosyl groups at their non-reducing termini.

from Flavobacterium
Product Number A1603 (powder, ≥10,000 units/g solid)

References
1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC4/2/2/3.html)

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Amylases, α-Glucosidase, Starches (Amylose and Amylopectin) and Glycogen



Polymers of α-(1-4)-D-glycopyranosyl units. Amylopectin has approximately 4% α-(1-6)-D-glycopyranosyl branching. Glycogen tends to have a higher frequency of branches of shorter length than amylopectin.

α-Amylase
EC# 3.2.1.1
Synonyms: 1,4-alpha-D-glucan glucanohydrolase

α-Amylase catalyzes the endohydrolysis of 1,4-α-D-glucosidic linkages in polysaccharides containing three or more 1,4-α-linked D-glucose units.

from Bacillus species
Product Number A6814 (powder, ≥400 units/mg protein (Lowry))
Product Number A6380 (Type II-A, lyophilized powder, ≥1,500 units/mg protein (biuret))

from Bacillus amyloliquefaciens
Product Number A7595 (liquid, ≥250 units)

from Bacillus licheniformis
Product Number A4551 (lyophilized powder, 500-1,500 units/mg protein, 93-100% (SDS-PAGE))
Product Number A3403 (Type XII-A, saline solution, ≥500 units/mg protein (biuret))
Product Number A3306 (solution, for use in Total Dietary Fiber Assay, TDF-100A)

from Aspergillus oryzae
Product Number 10065 (powder, ~30 U/mg)
Product Number 86250 (powder, ~1.5 U/mg (~0.2 U acc. to Willstätter)
Product Number A9857 (≥150 units/mg protein (biuret)
Product Number 86247 (powder, slightly beige, ~100 U/mg)
Product Number A8220 (aqueous solution, ≥800 FAU/g)

from Barley malt
Product Number A2771 (min 1 un/mg, lyophilized powder containing lactose)

from Porcine pancreas
Product Number A3176 (Type VI-B, ≥5 units/mg solid)
Product Number A6255 (DFP Treated, Type I-A, saline suspension, ≥1000 units/mg protein (E1%/280))
Product Number A4268 (Type I-A, PMSF treated, saline suspension, 700-1400 units/mg protein (E1%/280)

from Human pancreas
Product Number A9972 (lyophilized powder, ≥100 units/mg protein)

from Human saliva
Product Number A1031 (Type XIII-A, lyophilized powder, 300-1,500 units/mg protein)
Product Number A0521 (Type IX-A, lyophilized powder, 1,000-3,000 units/mg protein)

References
1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/1.html/)

β-Amylase
EC# 3.2.1.2
Synonyms: 1,4-α-D-glucan maltohydrolase

β-Amylase catalyzes the exo-hydrolysis of 1,4-α-D-glucosidic linkages in polysaccharides resulting in the successive liberation of maltose units from the non-reducing ends of the chains.

from Barley
Product Number A7130 (Type II-B, 20-80 units/mg protein (biuret))

from Sweet potato
Product Number A8781 (Gel filtration molecular weight marker, vial of 15 mg)
Product Number A7005 (Type I-B, ammonium sulfate suspension, ≥750 units/mg protein (E1%/280))

References
1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/2.html/

Amyloglucosidase
EC# 3.2.1.3
Synonyms: glucan 1,4-α-glucosidase, glucoamylase, γ-amylase

Amyloglucosidase catalyzes the hydrolysis of terminal 1,4-linked α-D-glucose residues successively from the non-reducing ends of maltooligo- and polysacharides with release of β-D-glucose. Most forms of the enzyme can rapidly hydrolyze 1,6-α-D-glucosidic bonds when the next bond in the sequence is 1,4- and some preparations of this enzyme hydrolyze 1,6- and 1,3-α-D-glucosidic bonds in other polysaccharides.

from Aspergillus niger
Product Number 10115 (lyophilized, powder, ~70 U/mg)
Product Number 10113 (powder, white, ~120 U/mg)
Product Number A7420 (lyophilized powder, 30-60 units/mg protein (biuret), ≤0.02% glucose)
Product Number A7095 (≥260 U/mL, aqueous solution)
Product Number A1602 (ammonium sulfate suspension, ≥40 units/mg protein)
Product Number ROAMYGLL (Lyophilized powder, approximately 6 U/mg lyophilizate)
Product Number ROAMYGL (Ammonium sulfate suspension, approximately  14 units/mg protein)

from Rhizopus species
Product Number A9228  

References
1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/3.html/)

α-Glucosidase
EC# 3.2.1.20
Synonyms: maltase

α-Glucosidase catalyzes the hydrolysis of terminal 1,4-linked α-D-glucose residues successively from the non-reducing ends of maltooligo- and to a lesser extent polysacharides with release of β-D-glucose. Most forms of the enzyme can slowly hydrolyze 1,6-α-D-glucosidic bonds.

from Bacillus stearothermophilus
Product Number G3651 (lyophilized powder, ≥50 units/mg protein)

from Saccharomyces cerevisiea
Product Number G0660 (recombinant, expressed in proprietary host, lyophilized powder, ≥100 units/mg protein)
Product Number G5003 (Type I, lyophilized powder, ≥10 units/mg protein (using p-nitrophenyl α-D-glucoside as substrate.)

from Rice
Product Number G9259 (Type V, ammonium sulfate suspension, 40-80 units/mg protein)

References
1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/20.html/)

Isoamylase
EC# 3.2.1.68
Synonyms: glycogen a-1,6-glucanohydrolase, debranching enzyme

from Pseudomonas sp.
Product Number 8124 (ammonium sulfate suspension, ≥10,000,000 units/mg protein)

Isoamylase catalyzes the hydrolysis of (1-6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins.

References
1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/68.html/)

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Cellulase, Lyticase, Laminanrinase, Cellulose, β-Glucans and Lichenen

Repeating linear polymer of two β-(1-4)-D-glycopyranosyl and one β-(1-3)-D-glycopyranosyl unit.

 

Polymer of β-(1-4)-D-glycopyranosyl units occurring as predominantly as cellotriose and cellotetraose separated by single β-(1-3)-D-glycopyranosyl units. Cross-linking can occur within the consecutive cellotriose regions.

 

Polymer of β-(1-3)-D-glycopyranosyl units with branching at β-(1-6)-D-glycopyranosyl units.

 

Cellulase
EC# 3.2.1.4
Synonyms: 1,4-(1,3;1,4)-β-D-glucan 4-glucanohydrolase

from Aspergillus species
Product Number C2605 (aqueous solution)

from Aspergillus niger
Product Number 22178 (powder, off-white, ~0.8 U/mg)
Product Number C1184 (powder, ≥0.3 units/mg solid)

from Trichoderma reesei
Product Number C8546 (lyophilized powder, ≥1 unit/mg solid)
Product Number C2730 (aqueous solution, ≥700 units/g)

from Trichoderma viride
Product Number C0615 (powder, ≥5,000 units/g solid)
Product Number C1794 (BioReagent, suitable for plant cell culture, 3-10 units/mg solid)
Product Number C9422 (powder, min. 3.0-10 un/mg)

Cellulase catalyzes the endohydrolysis of 1,4-β-D-glucosidic linkages in cellulose, lichenin and cereal β-D-glucans1

Cellobiohydrolase I
from Hypocrea jecorina.
Product Number E6412 (0.13 U/mg, recombinant, expressed in corn)

Driselase
from Basidiomycetes sp.
Product Number D9515 (powder, Protein ≥10 % by biuret)

Laminarinase
EC# 3.2.1.6
Synonyms: endo-1,3(4)-β-glucanase

Laminarinase catalyzes the endohydrolysis of 1,3- or 1,4-linkages in β-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolysed is itself substituted at C-3.

from Penicillium Species
Product Number L9295 (lyophilized powder, 5-10 un/mg)

Lyticase
Synonyms: Zymolyase
from Arthrobacter luteus
Product Number SAE0098 (free of DNA contaminants, suitable for Microbiome research, ≥2000 units/mg protein, lyophilized powder)
Product Number L2524 (lyophilized powder, ≥2,000 units/mg protein, Protein ≥20 % by biuret)
Product Number L4025 (lyophilized powder, ≥200 units/mg solid)
Product Number SAE0098 (lyophilized powder, min 4,000 un/mg protein)

References
1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/4.html)
2. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/6.html)

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