Tissue Dissociation and Cell Detachment Enzymes

Tissue Dissociation and Cell Detachment Enzymes

Select the ideal enzyme for your cell detachment or tissue dissociation needs, including trypsin, collagenase, papain, nucleases, hyaluronidase, elastase, and protease XIV. With the appropriate enzyme for treating your cellular target, you can maximize the yield of viable cells. Click below to learn about the factors that influence your tissue dissociation and discover optimal conditions for working with these cell detachment enzymes.

 

Trypsin
Serine protease commonly used for cell detachment and tissue dissociation.
Hyaluronidase
Catalyzes the random hydrolysis of 1,4-β-D-glycosidic linkages.
Collagenase
Because of its unique ability to hydrolyze native collagen, it is widely used to isolate cells from animal tissue.
Elastase
Useful for the dissociation of tissues containing higher amounts of elastin.
Papain
Relatively nonspecific sulfhydryl protease that can be used alone for dissociation.
Protease Type XIV
Extremely nonspecific protease for dissociation of various tissues.
Nucleases (DNase and RNase)
Reduces viscosity from DNA released from damaged cells during harvesting.