SigMatrix Serum Diluent and Urine Diluent Reagents

 Serum and urine diluents for ELISA sample dilution and LC-MS assays

Accurate calibration and quantitation is highly important for analysis of human samples.  Historically, mammalian derived materials such as serum, plasma, or urine have been used as diluents for samples and calibration controls in ELISA and LC-MS based assays. Ideally, a matrix for such applications would be devoid of any interfering substances, while having a defined composition similar to the target specimen. Naturally derived matrix materials are typically pooled from multiple donors and “stripped” to remove endogenous contaminants. The sourcing and removal processes of these materials often result in significant variation of composition between batches, leading to repetitive evaluation and labor-intensive lot qualifications for established systems.         

SigMatrix diluents are aqueous surrogate matrices that simulate conventional biological fluids while being devoid of most clinically relevant analytes that are often found in native matrix materials.  Manufactured with consistency and quality in mind, SigMatrix diluents provide alternatives to human and animal serum and urine products for drug, metabolite, and protein analysis. 

SigMatrix Serum Diluent and Urine Diluent ReagentsSigMatrix is:

  • Made of well-defined, stable materials
  • Ready-to-use formulation
  • Consistent from lot-to-lot
  • Produced from animal-free components
  • Absent of potentially infectious agents


SigMatrix Serum Diluent and Urine Diluent Product Comparison Table
 

Product Composition/Form Applications Quality Level Configuration
D5322 6% SigMatrix Serum Diluent   6% recombinant HSA (Human Serum Albumin) in PBS solution, pH 7.4, 0.01% ProClin™ 950   Recommended as a diluent for proteinaceous analytes in LC-MS/MS calibrators, controls, or samples   GMP - Reagents designed and manufactured under cGMP controls suitable for use in an IVD application Aqueous solution in 50mL and 1L bottle sizes
D4197 SigMatrix Serum Diluent   2% recombinant HSA (Human Serum Albumin) in PBS solution, pH 7.4, 0.1% ProClin™ 950   Suitable for use in ELISA and small molecule LC-MS-based diagnostic applications IVD (for in-vitro diagnostic use) Aqueous solution in 100mL and 1L bottle sizes
D5197 SigMatrix Ultra Serum Diluent   Ultra-pure, preservative free 2% recombinant HSA (Human Serum Albumin) in PBS solution, pH 7.4   Recommended for the most sensitive small molecule LC-MS-based diagnostic applications GMP - Reagents designed and manufactured under cGMP controls suitable for use in an IVD application Aseptically filled aqueous solution in 100mL, 5 X 100mL, and 500mL bottle sizes  
SAE0074 SigMatrix Urine Diluent   Synthetic urine containing calcium chloride, magnesium chloride, potassium chloride, sodium chloride, sodium phosphate, sodium sulfate, urea, and creatinine with sodium azide   Suitable for ELISA, sample prep, use in enzymatic hydrolysis of drug glucuronides, and LC-MS analysis of drugs and drug metabolites in urine   Research use only.  Not for use in diagnostic procedures Aqueous solution in a 1L bottle size

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