High Quality DNA for Automated Sequencing

Electropherogram revealing >600 bases of sequence from pUC-TMV using GenElute Plasmid Miniprep Kit. Cycle Sequencing was performed using 500 ng of template, a T7 sequencing primer, and ABI Big DyeTM terminator chemistry. Sequencing reactions were resolved on an ABI Prism 377 XL instrument with a 48 cm gel cassette containing 4.5% AutoPAGE Plus acrylamide at 2.4 kV, 48°C for 7 hours.

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