DNA & RNA Purification

SYBR® Green Extract-N-AmpTissue Kit


Description: The SYBR® Green Extract-N-AmpTissue PCR Kit contains all the reagents needed for rapid extraction, amplification and detection of genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.

  • Novel – all liquid, single-step extraction of genomic DNA for quantitative PCR (qPCR)
  • Fast – tissue to qPCR in 15 minutes
  • Convenient – no long enzymatic digestions and no column purifications
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The SYBR® Green Extract-N-Amp™ Tissue PCR Kit offers an innovative extraction system that eliminates the need for either long enzymatic digestions or homogenization. The product includes a specially formulated Hot Start SYBR® Green PCR ReadyMix™ for amplification and quantitation directly from the extract.

Procedure: DNA is rapidly extracted from a tissue by incubating the sample with a mixture of the Extraction Solution and the Tissue Preparation Solution at room temperature for 10 minutes. After a 3-minute heat denaturing step, an equal volume of Neutralization Solution B is added to the extract to neutralize inhibitory substances. The extract is ready for real-time PCR in any plate-based real-time thermal cycling system in less than 15 minutes.

Application: Ideal for genotyping, gene copy number experiments, and amplifying and quantifying DNA from multiple tissue sample types.

Watch the video protocol Rapid Genotyping of Mouse Tissue Using Extract-N-Amp™ Tissue PCR Kit


Watch the full video protocol here
Video brought to you by JoVE.com



Incubate with Extraction Solution for 10 minutes at room temperature.

Heat at 95°C for 3 minutes.



Add Neutralization Solution.



Mix aliquot with Extract-N-Amp™ SYBR® Green PCR ReadyMix™ and primers.


Transfer to thermal cycler.

PCR.

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Beneficial Features


 

Speed From tissue to qPCR in less than 15 minutes
Convenient No long enzymatic digestions needed.
Simple Rapid, easy to follow protocol.
Sensitive Specially formulated Hot Start SYBR® Green PCR ReadyMix™ for highly specific PCR amplification and quantitation of genomic DNA.
Safe No organic extraction with hazardous chemicals.

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Order Information


 

Product Code Product Name Number of Extractions Number of Amplifications Technical Bulletin.pdf
XNATG SYBR® Green Extract-N-Amp™ Tissue PCR Kit 100 100 TB
XNATRG SYBR® Green Extract-N-Amp™ Tissue PCR Kit 1000 1000 TB

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Distinguish differences in gene copy number


 

SYBR Green Extract-N-Amp Distinguishes Between 1-2 Gene Copies

Figure 1. SYBR® Green Extract-N-Amp™ Used to Distinguish between 1-2 Gene Copies. Extracts were prepared following the standard protocol for the SYBR® Green Extract-N-Amp™ Tissue PCR Kit from 8 mouse tails that contained either a single or double copy of the Diap2 gene. Two single-gene PCR reactions were run on each extract using the 2x Extract-N-Amp™ SYBR® Green PCR ReadyMix.

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Flexible enough for a wide variety of tissue types


 

SYBR Green Extract-N-Amp PCR Data for a Variety of Tissue Types

 

Figure 2. Quantitative PCR of DNA isolated from a variety of tissue types and sources. The Extract-N-Amp™ Tissue PCR Kit was used to extract and amplify genomic DNA from various sources. The extracted DNA was then amplified using the specially formulated Hot Start SYBR® Green PCR ReadyMix.

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Highly sensitive SYBR® Green PCR ReadyMix™


 

Quantitative PCR of DNA

 

Figure 3. Quantitative PCR of DNA isolated from a series of decreasing extract dilutions. DNA was extracted from a mouse tail snip following the standard protocol for the SYBR® Green Extract-N-Amp™ Tissue PCR Kit. The extract was diluted in decreasing three-fold increments, from 100% extract to 0.14% extract. As depicted above, message can be clearly detected even with the most dilute extract.

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