CompoZr® Knockout ZFN Kits

CompoZr® Knockout ZFNs create targeted double strand breaks at a gene specific target locus. Through the cellular process of non-homologous end joining, this double strand break can result in modification of the DNA sequence and therefor create a functional knockout of the targeted gene. To provide the best chance of creating a functional gene knockout, CompoZr Knockout ZFNs are targeted to the first 2/3 of the coding region for the gene of interest.

CompoZr Knockout ZFNs are provided as two plasmids encoding for the gene specific zinc finger nuclease and 10 aliquots of mRNA encoding for the ZFN. Also included in the kit are custom-designed primers, that allow for determination of the rate of mutation and for screening of individual clones that have the desired mutational event, and positive control DNA that has been modified using the CompoZr Knockout ZFN.

All Zinc Finger Nucleases are sold under license from Sangamo Biosciences. For a copy of the Label License provided with purchase of CompoZr ZFNs, please visit the ZFN Label License page. CompoZr is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.