Fast QPCR

Sigma Life Science offers two product lines for fast qPCR: KiCqStart qPCR ReadyMixes and the LuminoCt qPCR Reagents. Both mixes offer the convenience of a 2X concentrated mix which includes an antibody-mediated hot-start Taq DNA polymerase, dNTPs, MgCl2, and optimized buffers for fast qPCR. Depending on your thermal cycler, you can reduce your real-time PCR run times from 2 hours to 25 minutes!

Additionally, we also offer ABScript II One Step RT-qPCR Probe Kit, a one-step RT-qPCR kit that is compatible with all major qPCR instruments. This kit is ideal for analyzing low input RNA samples.

The KiCqStart qPCR ReadyMix is available in four different formulations to offer the additional convenience of having the passive reference dye, if used by your real-time PCR instrument, already mixed into the solution.

The LuminoCt Fast qPCR reagents are available for both SYBR Green and Probe-based real-time PCR applications. LuminoCt offers the flexibility of a formula that can be used on any real-time PCR instrument. A separate vial of ROX passive reference dye is provided so that it may be added into the reactions if required by your real-time PCR platform.

Learn more about KiCqStart ReadyMixes
Learn more about LuminoCt ReadyMixes

ABScript II One Step RT-qPCR Probe Kit

The ABScript II One Step RT-qPCR Probe Kit is a ready-to-use kit for reverse transcription and subsequent probe-based qPCR in a single tube. This kit′s flexible format is compatible with all major qPCR instruments. It includes two separate 50X concentrations of the ROX (passive reference) dye, to be added as required by specific qPCR instruments.

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RK20407 ABScript II One Step RT-qPCR Probe Kit

KiCqStart® qPCR ReadyMix

KiCqStart...qPCR Mixes Optimized for your qPCR instrument.
In addition to our KicqStart SYBR qPCR ReadyMixes, Sigma now introduces Readymixes for probe based qPCR and qRT-PCR. These new products have the same great benefits of our KicqStart SYBR mixes, including:

  • Assay results in as little as 33 minutes
  • Highly efficient and sensitive real-time PCR results
  • Robust formulation requiring little to no optimization

Each KiCqStart mix is a specially-formulated 2X concentrate with an antibody-based hot-start Taq DNA polymerase and all the optimized components needed for fast, reliable, and reproducible qPCR assays. The qRT-PCR formulation also has reverse transcriptase and RNase inhibitor build into the mix.

Select your ReadyMix below to learn more.

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KCQS07 KiCqStart® One-Step Probe RT-qPCR ReadyMix for Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems
KCQS08 KiCqStart® One-Step Probe RT-qPCR ReadyMix, Low ROX with Low ROX for ABI and Stratagene instruments
KCQS09 KiCqStart® One-Step Probe RT-qPCR ReadyMix, ROX with ROX for ABI instruments
KCQS04 KiCqStart® Probe qPCR ReadyMix for Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems
KCQS05 KiCqStart® Probe qPCR ReadyMix, Low ROX with Low ROX for ABI and Stratagene instruments
KCQS06 KiCqStart® Probe qPCR ReadyMix, ROX with ROX for ABI instruments
KCQS00 KiCqStart® SYBR® Green qPCR ReadyMix For Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems
KCQS01 KiCqStart® SYBR® Green qPCR ReadyMix Low ROX, for ABI and Stratagene instruments
KCQS02 KiCqStart® SYBR® Green qPCR ReadyMix with ROX for ABI instruments
KCQS03 KiCqStart® SYBR® Green qPCR ReadyMix iQ, with fluorescein for Bio-Rad systems

LuminoCt® qPCR ReadyMix

The LuminoCt Fast qPCR reagents are available for both SYBR Green and Probe-based real-time PCR applications. LuminoCt offers the flexibility of a formula that can be used on any real-time PCR instrument. A separate vial of ROX passive reference dye is provided so that it may be added into the reactions if required by your real-time PCR platform.

Learn more about LuminoCt

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Description

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L6669 LuminoCt® qPCR ReadyMix For fast probe-based quantitative PCR
L6544 LuminoCt® SYBR® Green qPCR ReadyMix For fast SYBR Green quantitative PCR