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Fast QPCR

Sigma Life Science offers two product lines for fast qPCR: KiCqStart qPCR ReadyMixes and the LuminoCt qPCR Reagents. Both mixes offer the convenience of a 2X concentrated mix which includes an antibody-mediated hot-start Taq DNA polymerase, dNTPs, MgCl2, and optimized buffers for fast qPCR. Depending on your thermal cycler, you can reduce your real-time PCR run times from 2 hours to 25 minutes!

The KiCqStart qPCR ReadyMix is available in four different formulations to offer the additional convenience of having the passive reference dye, if used by your real-time PCR instrument, already mixed into the solution.

The LuminoCt Fast qPCR reagents are available for both SYBR Green and Probe-based real-time PCR applications. LuminoCt offers the flexibility of a formula that can be used on any real-time PCR instrument. A separate vial of ROX passive reference dye is provided so that it may be added into the reactions if required by your real-time PCR platform.

Learn more about KiCqStart ReadyMixes

Learn more about LuminoCt ReadyMixes

KiCqStart® qPCR ReadyMix

KiCqStart...qPCR Mixes Optimized for your qPCR instrument.
In addition to our KicqStart SYBR qPCR ReadyMixes, Sigma now introduces Readymixes for probe based qPCR and qRT-PCR. These new products have the same great benefits of our KicqStart SYBR mixes, including:

  • Assay results in as little as 33 minutes
  • Highly efficient and sensitive real-time PCR results
  • Robust formulation requiring little to no optimization

Each KiCqStart mix is a specially-formulated 2X concentrate with an antibody-based hot-start Taq DNA polymerase and all the optimized components needed for fast, reliable, and reproducible qPCR assays. The qRT-PCR formulation also has reverse transcriptase and RNase inhibitor build into the mix.

Select your ReadyMix below to learn more.

Product #

Description

Add to Cart

KCQS07 KiCqStart® One-Step Probe RT-qPCR ReadyMix for Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems
KCQS08 KiCqStart® One-Step Probe RT-qPCR ReadyMix, Low ROX with Low ROX for ABI and Stratagene instruments
KCQS09 KiCqStart® One-Step Probe RT-qPCR ReadyMix, ROX with ROX for ABI instruments
KCQS04 KiCqStart® Probe qPCR ReadyMix for Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems
KCQS05 KiCqStart® Probe qPCR ReadyMix, Low ROX with Low ROX for ABI and Stratagene instruments
KCQS06 KiCqStart® Probe qPCR ReadyMix, ROX with ROX for ABI instruments
KSPQ12012 KiCqStart® SYBR® Green Primers Predesigned primers for gene expression analysis
KSGA2013 KiCqStart® SYBR® Green Primers Gene Arrays Primer arrays for expression analysis of gene families
KCQS00 KiCqStart® SYBR® Green qPCR ReadyMix For Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems
KCQS01 KiCqStart® SYBR® Green qPCR ReadyMix Low ROX, for ABI and Stratagene instruments
KCQS02 KiCqStart® SYBR® Green qPCR ReadyMix with ROX for ABI instruments
KCQS03 KiCqStart® SYBR® Green qPCR ReadyMix iQ, with fluorescein for Bio-Rad systems

LuminoCt® qPCR ReadyMix

The LuminoCt Fast qPCR reagents are available for both SYBR Green and Probe-based real-time PCR applications. LuminoCt offers the flexibility of a formula that can be used on any real-time PCR instrument. A separate vial of ROX passive reference dye is provided so that it may be added into the reactions if required by your real-time PCR platform.

Learn more about LuminoCt

Product #

Description

Add to Cart

L6669 LuminoCt® qPCR ReadyMix For fast probe-based quantitative PCR
L6544 LuminoCt® SYBR® Green qPCR ReadyMix For fast SYBR Green quantitative PCR