ICAT

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  • The ICAT® (Isotope Coded Affinity Tag) reagents utilize cysteine-reactive, stable isotope-labeled compounds with a cleavable biotin group for affinity capture.
  • Capture of cysteine-tagged ICAT® peptides simplifies the peptide pool for MS analysis, while maintaining near-complete coverage of the total protein population.
  • Parallel sample states can be compared (or “duplexed”) with the ICAT® reagents.
  • Related Documents:
Protocol for the Cleavable ICAT Reagent Kit for Protein Labeling (monoplex version)
Protocol for the 1-D PAGE Cleavable ICAT Reagent Applications Development Kit for Targeted Protein ID and Quantitation (monoplex version)
Quick Reference Card for the Cleavable ICAT Reagent Kit for Protein Labeling
Quick Reference Procedure for the 1-D PAGE Cleavable ICAT Reagent Applications Development Kit for Targeted Protein ID and Quantitation (monoplex version)
References:
1. Hansen, K.C., et al., Mass Spectrometric Analysis of Protein Mixtures at Low Levels Using Cleavable 13C-Isotope-coded Affinity Tag and Multidimensional Chromatography. Mol. Cell. Proteomics, 2(5), 299-314 (2003). Abstract
2. Wu, C., et al., Distinction of thioredoxin transnitrosylation and denitrosylation target proteins by the ICAT quantitative approach. J. Proteomics, 74(11), 2498-2509 (2011). Abstract
3. Romero, D., et al., Endoglin Regulates Cancer-Stromal Cell Interactions in Prostate Tumors. Cancer Res., 71(10), 3482-3493 (2011). Abstract
4. Sun, Q.-A., et al., Oxygen-coupled Redox Regulation of the Skeletal Muscle Ryanodine Receptor/Ca2+ Release Channel (RyR1): Sites and Nature of Oxidative Modification. J. Biol. Chem., 288(32), 22961-22971 (2013). Abstract
5. Lindemann, C., et al., Redox Proteomics Uncovers Peroxynitrite-sensitive Proteins That Help Escherichia coli to Overcome Nitrosative Stress. J. Biol. Chem., 288(27), 19698-19714 (2013). Publication
6. Go, Y.-M., et al., Selective Targeting of the Cysteine Proteome by Thioredoxin and Glutathione Redox Systems. Mol. Cell. Proteomics, 12(11), 3285-3296 (2013). Abstract
7. Fu, C., et al., Identification of thioredoxin target protein networks in cardiac tissues of a transgenic mouse. Methods Mol. Biol., 1005, 181–197 (2013). Publication
8. Garcia-Santamarina, S., et al., Monitoring in vivo reversible cysteine oxidation in proteins using ICAT and mass spectrometry. Nat. Protoc., 9(5), 1131-1145 (2014). Abstract
9. Favreau, A.J., et al., Cryptic collagen IV promotes cell migration and adhesion in myeloid leukemia. Cancer Med., 3(2), 265-272 (2014). Abstract
10. Beuve, A., et al., Identification of novel S-nitrosation sites in soluble guanylyl cyclase, the nitric oxide receptor. J. Proteomics, 138, 40-47 (2016). Abstract
11. Yamada, N., "Mass Spectrometry Utilizing Isotope-Coded Affinity Tag Reagents", in Quantitative Proteome Analysis: Methods and Applications (Kazuhiro Imai and Sam F.Y. Li, editors). CRC Press/Taylor & Francis Group,.ISBN 978-981-4316-51-4, pp. 35-43 (2013).