Duolink PLA Technology

Proximity ligation assay (PLA) to detect protein interaction

Comprehensive Protein Analysis with Duolink PLA Products

Detect, quantify and determine cell localization of protein interactions and their modifications in a single experiment. With signal amplification up to 1000 times, Duolink proximity ligation assay (PLA) technology allows for protein detection under endogenous protein expression levels.

Two primary antibodies (PLA, IHC, ICC or IF validated) from different species that recognize two target epitopes must be selected before using Duolink. Other necessary reagents to run a Duolink experiment include a pair of PLA probes (one PLUS and one MINUS) with hosts corresponding to those of the primary antibodies, detection reagents, wash buffers, and a mounting medium. Use our Product Selection Guide to help you determine the best Duolink products for your experiment.

For more information about this technology, visit our Duolink PLA Resource Center

Primary Antibodies for use with Duolink
Browse our selection of PLA-, IHC-, and/or IF-validated antibodies, pre-qualified for use with Duolink

Duolink Starter Kits

The Duolink starter kit contains all components necessary to analyze up to 30 reactions in one single order. Select one, from the six available below, based on the primary antibody species and desired detection color:

Product #

Description

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DUO92101 Duolink In Situ Red Starter Kit Mouse/Rabbit
DUO92102 Duolink In Situ Orange Starter Kit Mouse/Rabbit
DUO92103 Duolink In Situ Red Starter Kit Mouse/Goat
DUO92104 Duolink In Situ Orange Starter Kit Mouse/Goat
DUO92105 Duolink In Situ Red Starter Kit Goat/Rabbit
DUO92106 Duolink In Situ Orange Starter Kit Goat/Rabbit

Duolink PLA Probes

Product #

Description

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DUO92001 Duolink In Situ PLA® Probe Anti-Mouse PLUS
DUO92002 Duolink In Situ PLA® Probe Anti-Rabbit PLUS Affinity purified Donkey anti-Rabbit IgG (H+L)
DUO92003 Duolink In Situ PLA® Probe Anti-Goat PLUS
DUO92004 Duolink In Situ PLA® Probe Anti-Mouse MINUS Affinity purified Donkey anti-Mouse IgG (H+L)
DUO92005 Duolink In Situ PLA® Probe Anti-Rabbit MINUS
DUO92006 Duolink In Situ PLA® Probe Anti-Goat MINUS Affinity purified Donkey anti-Goat IgG (H+L)
DUO92009 Duolink In Situ Probemaker PLUS
DUO92010 Duolink In Situ Probemaker MINUS
DUO92020 Duolink In Situ PLA® Probe Anti-Human PLUS Affinity purified Donkey anti-Human IgG (H+L)
DUO92021 Duolink In Situ PLA® Probe Anti-Human MINUS Affinity purified Donkey anti-Human IgG (H+L)

Duolink PLA Detection Reagents

Product #

Description

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DUO92007 Duolink In Situ Detection Reagents Orange
DUO92008 Duolink In Situ Detection Reagents Red
DUO92012 Duolink In Situ Detection Reagents Brightfield
DUO92013 Duolink In Situ Detection Reagents FarRed
DUO92014 Duolink In Situ Detection Reagents Green

Duolink PLA Accessories

Product #

Description

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DUO82040 Duolink In Situ Mounting Medium with DAPI
DUO82047 Duolink In Situ Wash Buffer, Brightfield
DUO82049 Duolink In Situ Wash Buffers, Fluorescence
DUO82064 Duolink In Situ Microplate Nuclear Stain, Anti-Fade
DUO82065 Duolink In Situ Microplate Heat Transfer Block
DUO92202 Duolink PLA Control Kit - PPI Control Kit to reliably detect Protein-Protein Interactions (PPI) with Duolink PLA

Duolink flowPLA Detection Kits

Product #

Description

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DUO94001 Duolink flowPLA Detection Kit - Red DUOLINK : PLA kit for Flow Cytometry with Red Detection
DUO94002 Duolink flowPLA Detection Kit - Green Duolink PLA kit for Flow Cytometry with Green Detection
DUO94003 Duolink flowPLA Detection Kit - Orange Duolink PLA kit for Flow Cytometry with Orange Detection
DUO94004 Duolink flowPLA Detection Kit - FarRed Duolink PLA kit for Flow Cytometry with FarRed Detection
DUO94005 Duolink flowPLA Detection Kit - Violet Duolink PLA kit for Flow Cytometry with Violet Detection

Duolink flowPLA Starter Kits

Product #

Description

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DUO94102 Duolink flowPLA Mouse/Rabbit Starter Kit - Green Duolink PLA kit for Flow Cytometry with Green Detection and Mouse/Rabbit probes New
DUO94104 Duolink flowPLA Mouse/Rabbit Starter Kit - Far Red Duolink PLA kit for Flow Cytometry with Far Red Detection and Mouse/Rabbit probes New
DUO94105 Duolink flowPLA Mouse/Rabbit Starter Kit - Violet Duolink PLA kit for Flow Cytometry with Violet Detection and Mouse/Rabbit probes New

Duolink PLA Multicolor Kits

Product #

Description

Add to Cart

DUO96000 Duolink PLA Multicolor Reagent Pack
DUO96010 Duolink PLA Multicolor Probemaker Kit - Red Proximity Ligation Assay Multiplexing: protein-protein interaction, post-translational modification and low abundant protein events
DUO96020 Duolink PLA Multicolor Probemaker Kit - Green Proximity Ligation Assay Multiplexing: protein-protein interaction, post-translational modification and low abundant protein events
DUO96030 Duolink PLA Multicolor Probemaker Kit - Orange Proximity Ligation Assay Multiplexing: protein-protein interaction, post-translational modification and low abundant protein events
DUO96040 Duolink PLA Multicolor Probemaker Kit - Far Red Proximity Ligation Assay Multiplexing: protein-protein interaction, post-translational modification and low abundant protein events