Millipore® PVDF Membranes for Western Blotting

We offer both PVDF and nitrocellulose membranes for Western blotting, as well as blotting sandwiches that feature pre-cut sheets of membrane and blotting filter paper.

PVDF membranes offer several clear advantages over nitrocellulose membranes
  • Won′t crack or curl, and they can be cut without fracturing
  • Low background
  • Broad solvent compatibility
  • Superior staining capabilities and suitable for reprobing
  • Compatible with a variety of detection chemistries


Immobilon®-P Membranes: 0.45 μm PVDF membranes optimized for Western blotting

Immobilon®-E Membranes:First transfer membrane that can be wet-out in buffer or water, eliminating the need for a pre-wet step with alcohol

Immobilon®-PSQ Membranes: PVDF membrane with greater protein binding capacity

Immobilon®-FL Membranes:</B> First transfer membrane optimized for fluorescence applications

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IESN07852 Immobilon-E Blotting Sandwich 7 x 8.4 cm, Immobilon-E membrane interleaved with blotting paper, for standard tank transfer procedures. PVDF membrane wets out in water or transfer buffer.
IESN08132 Immobilon-E Blotting Sandwich 8.5 x 13.5 cm, Immobilon-E membrane interleaved with blotting paper,for standard tank transfer procedures. PVDF membrane wets out in water or transfer buffer.
IPSN07852 Immobilon-P Blotting Sandwich 7 x 8.4 cm, Immobilon-P membrane interleaved with blotting paper, for standard tank transfer procedures
IPSN08132 Immobilon-P Blotting Sandwich 8.5 x 13.5 cm, Immobilon-P membrane interleaved with blotting paper,for standard tank transfer procedures
IPFL85R Immobilon-FL PVDF, 0.45 µm, 8.5 cm x 10 m roll 0.45 um ( 0.45 micron filter ) pore size Hydrophobic PVDF Transfer Membrane for western blotting using Fluorescent, Chemifluorescence, Chromogenic, or Chemiluminescence. Comes in a 8.5 cm x 10 m roll.
IPFL07810 Immobilon-FL PVDF Membrane 10 sheets, 7 cm x 8.4 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.
IPFL20200 Immobilon-FL PVDF Membrane 10 sheets, 20 cm x 20 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.
IPFL00010 Immobilon-FL PVDF Membrane 1 roll, 26.5 cm x 3.75 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.
IPFL00005 Immobilon-FL PVDF Membrane 1 roll, 26.5 cm x 1.875 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.
IPFL10100 Immobilon-FL PVDF Membrane 10 sheets, 10 cm x 10 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.
IPVH85R Immobilon-P Membrane, PVDF, 0.45 µm, 8.5 cm x 10 m roll 0.45 um ( 0.45 micron filter ) pore size Hydrophobic immobilon-P PVDF Transfer Membrane for western blotting using Chemiluminescence, Chromogenic or Radioactive detection methods. Comes in an 8.5 cm x 10 m roll.
N6645 Immobilon®-NC HAHY membranes L × W 10 ft × 1 ft
IMDISP Immobilon NOW Dispenser Dispenser for transfer membrane used in Western blotting, immunoblotting and dot blotting applications
ISEQ00005 Immobilon-PSQ PVDF Membrane 1 roll, 26.5 cm x 1.875 m, 0.2 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
ISEQ07850 Immobilon-PSQ PVDF Membrane 50 sheets, 7 cm x 8.4 cm, 0.2 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
ISEQ10100 Immobilon-PSQ PVDF Membrane 10 sheets, 10 cm x 10 cm, 0.2 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
ISEQ00010 Immobilon-PSQ PVDF Membrane 1 roll, 26.5 cm x 3.75 m, 0.2 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
ISEQ08130 Immobilon-PSQ PVDF Membrane 10 sheets, 8.5 cm x 13.5 cm, 0.2 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
ISEQ20200 Immobilon-PSQ PVDF Membrane 10 sheets, 20 cm x 20 cm, 0.2 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
ISEQ09120 Immobilon-PSQ PVDF Membrane 10 sheets, 9 cm x 12 cm, 0.2 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
ISEQ26260 Immobilon-PSQ PVDF Membrane 10 sheets, 26 cm x 26 cm, 0.2 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
ISEQ15150 Immobilon-PSQ PVDF Membrane 10 sheets, 15 cm x 15 cm, 0.2 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
ISEQ08100 Immobilon-PSQ PVDF Membrane 10 sheets, 8 cm x 10 cm, 0.2 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
IEVH08100 Immobilon-E PVDF Membrane 10 sheets, 8 cm x 10 cm, The first PVDF transfer Membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.
IEVH00005 Immobilon-E PVDF Membrane 1 roll, 26.5 cm x 1.875 m, The first PVDF transfer Membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.
IEVH07804 Immobilon-E PVDF Membrane 4 sheets, 7 cm x 8.4 cm, The first PVDF transfer Membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.
IEVH07850 Immobilon-E PVDF Membrane 50 sheets, 7 cm x 8.4 cm, The first PVDF transfer Membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.
IEVH85R Immobilon-E PVDF Membrane The first PVDF transfer Membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.
IEVH09120 Immobilon-E PVDF Membrane 10 sheets, 9 cm x 12 cm, The first PVDF transfer Membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.
IEVH10100 Immobilon-E PVDF Membrane 10 sheets, 10 cm x 10 cm, The first PVDF transfer Membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.
IPVH08100 Immobilon-P PVDF Membrane 10 sheets, 8 cm x 10 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
IPVH00010 Immobilon-P PVDF Membrane 1 roll, 26.5 cm x 3.75 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
IPVH09120 Immobilon-P PVDF Membrane 10 sheets, 9 cm x 12 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
IPVH10100 Immobilon-P PVDF Membrane 10 sheets, 10 cm x 10 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
IPVH08130 Immobilon-P PVDF Membrane 10 sheets, 8.5 cm x 13.5 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
IPVH07850 Immobilon-P PVDF Membrane 50 sheets, 7 cm x 8.4 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
IPVH304F0 Immobilon-P PVDF Membrane 10 sheets, 26 cm x 26 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
IPVH15150 Immobilon-P PVDF Membrane 10 sheets, 15 cm x 15 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
IPVH20200 Immobilon-P PVDF Membrane 10 sheets, 20 cm x 20 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
IPVH00005 Immobilon-P PVDF Membrane 1 roll, 26.5 cm x 1.875 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
ISEQ85R Immobilon-PSQ Membrane, PVDF, 0.2 µm, 8.5 cm x 10 m roll 0.2 um filter ( 0.2 micron ) pore size Hydrophobic PVDF Transfer Membrane for western blotting using Chemiluminescence, Chromogenic or Radioactive detection methods. Comes in an 8.5 cm x 10 m roll.
WBKLS Immobilon Western Chemiluminescent HRP Substrate Immobilon Western HRP Substrate provides high sensitivity for Chemiluminescent detection in western or dot/slot/spot blotting applications on both PVDF and nitrocellulose membranes, and is compatible with all commonly used buffers and blocking reagents.