Immobilon® PVDF Membranes for Western Blotting

MilliporeSigma offers three different PVDF membranes, each optimized for different protein blotting applications. We also now offer blotting sandwiches that feature pre-cut sheets of membrane and blotting filter paper.

Immobilon® PVDF Membrane Advantages Compared to Nitrocellulose
  • Won′t crack or curl, and they can be cut without fracturing.
  • Low background
  • Broad solvent compatibility
  • Superior staining capabilities
  • Can be reprobed multiple times
  • Compatible with a variety of detection chemistries, including radioactive, chromogenic, chemiluminescent, fluorescent, and chemifluorescent techniques

Immobilon® PVDF membranes offer excellent protein binding and high signal.
Immobilon PVDF membranes have high protein adsorption, so you won′t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon PVDF membranes optimized for fluorescent blots dramatically increase signal for high sensitivity in quantitative, multiplexing applications.

Immobilon-P PVDF Membranes: Immobilon-P PVDF (Polyvinylidene Difluoride) membranes have been optimized for Western blotting and offer better handling and staining than nitrocellulose. All Immobilon-P PVDF membranes have a pore size of 0.45 μm.

Immobilon-PSQ Membranes: PVDF with greater protein binding capacity than standard Immobilon-P. Thus, in protein sequencing applications, dramatically higher yields can be obtained. We recommend traditional Immobilon-P membranes for routine Western blotting applications. Immobilon-PSQ membranes are functional in these applications but will have higher background.

Immobilon-FL PVDF Membrane: The Immobilon-FL PVDF membrane is the first transfer membrane optimized for fluorescence applications. It demonstrates extremely low background, which improves sensitivity of all fluorescence detection protocols and is compatible with all commonly used fluorescent probes at all excitation and emission wavelengths. It is ideal for multiplexing and chemifluorescence applications.

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HATF00010 Immobilon-NC Transfer Membrane 1 roll, 33 cm x 3 m, 0.45 µm pore size, Triton-free, mixed cellulose esters transfer membrane
HATF01300 Immobilon-NC Transfer Membrane 13 mm diameter disc, 0.45 µm pore size, Triton-free, mixed cellulose esters transfer membrane
HATF02500 Immobilon-NC Transfer Membrane 25 mm diameter disc, 0.45 µm pore size, Triton-free, mixed cellulose esters transfer membrane
HATF04700 Immobilon-NC Transfer Membrane 47 mm diameter disc, 0.45 µm pore size, Triton-free, mixed cellulose esters transfer membrane
HATF08250 Immobilon-NC Membrane, Triton-free, mixed cellulose esters, 0.45 µm, 82 mm, disc 0.45 um ( 0.45 micron filter ) pore size Mixed Cellulose Esters, Surfactant-Free, Transfer Membrane for western blotting using Chromogenic, Radioactive or Fluorescent detection methods.
HATF08550 Immobilon-NC Membrane, Triton-free, mixed cellulose esters, 0.45 µm, 85 mm, disc 0.45 um ( 0.45 micron filter ) pore size Mixed Cellulose Esters, Surfactant-Free, Transfer Membrane for western blotting using Chromogenic, Radioactive or Fluorescent detection methods.
HATF09025 Immobilon-NC Transfer Membrane 90 mm diameter disc, 0.45 µm pore size, Triton-free, mixed cellulose esters transfer membrane
HATF10050 Immobilon-NC Transfer Membrane 100 mm diameter disc, 0.45 µm pore size, Triton-free, mixed cellulose esters transfer membrane
HATF14250 Immobilon-NC Transfer Membrane 142 mm diameter disc, 0.45 µm pore size, Triton-free, mixed cellulose esters transfer membrane
IBFP0785C Immobilon Blotting Filter Paper 7 x 8.4 cm sheet, Chromotography-Grade Blotting Paper for western blotting.
IBFP0813C Immobilon Blotting Filter Paper 8.5 x 13.5 cm sheet, Chromotography-Grade Blotting Paper for western blotting.
IESN07852 Immobilon-E Blotting Sandwich 7 x 8.4 cm, Immobilon-E membrane interleaved with blotting paper, for standard tank transfer procedures. PVDF membrane wets out in water or transfer buffer.
IESN08132 Immobilon-E Blotting Sandwich 8.5 x 13.5 cm, Immobilon-E membrane interleaved with blotting paper,for standard tank transfer procedures. PVDF membrane wets out in water or transfer buffer.
IEVH00005 Immobilon-E PVDF Membrane 1 roll, 26.5 cm x 1.875 m, The first PVDF transfer Membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.
IEVH07804 Immobilon-E PVDF Membrane 4 sheets, 7 cm x 8.4 cm, The first PVDF transfer Membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.
IEVH07850 Immobilon-E PVDF Membrane 50 sheets, 7 cm x 8.4 cm, The first PVDF transfer Membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.
IEVH08100 Immobilon-E PVDF Membrane 10 sheets, 8 cm x 10 cm, The first PVDF transfer Membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.
IEVH09120 Immobilon-E PVDF Membrane 10 sheets, 9 cm x 12 cm, The first PVDF transfer Membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.
IEVH10100 Immobilon-E PVDF Membrane 10 sheets, 10 cm x 10 cm, The first PVDF transfer Membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.
INYC00010 Immobilon-Ny+ Membrane, charged Nylon, 0.45 µm, 30 cm x 3.3 m roll Web-Supported Positively Charged Nylon Transfer Membrane for western blotting with Chemiluminescence, Radioactive & Fluorescent detection methods. Comes in a 30 x 330 sheet.
IPFL00005 Immobilon-FL PVDF Membrane 1 roll, 26.5 cm x 1.875 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.
IPFL00010 Immobilon-FL PVDF Membrane 1 roll, 26.5 cm x 3.75 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.
IPFL07810 Immobilon-FL PVDF Membrane 10 sheets, 7 cm x 8.4 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.
IPFL10100 Immobilon-FL PVDF Membrane 10 sheets, 10 cm x 10 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.
IPFL20200 Immobilon-FL PVDF Membrane 10 sheets, 20 cm x 20 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.
IPSN07852 Immobilon-P Blotting Sandwich 7 x 8.4 cm, Immobilon-P membrane interleaved with blotting paper, for standard tank transfer procedures
IPSN08132 Immobilon-P Blotting Sandwich 8.5 x 13.5 cm, Immobilon-P membrane interleaved with blotting paper,for standard tank transfer procedures
IPVH00005 Immobilon-P PVDF Membrane 1 roll, 26.5 cm x 1.875 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
IPVH00010 Immobilon-P PVDF Membrane 1 roll, 26.5 cm x 3.75 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
IPVH07850 Immobilon-P PVDF Membrane 50 sheets, 7 cm x 8.4 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
IPVH08100 Immobilon-P PVDF Membrane 10 sheets, 8 cm x 10 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
IPVH08130 Immobilon-P PVDF Membrane 10 sheets, 8.5 cm x 13.5 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
IPVH09120 Immobilon-P PVDF Membrane 10 sheets, 9 cm x 12 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
IPVH10100 Immobilon-P PVDF Membrane 10 sheets, 10 cm x 10 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
IPVH15150 Immobilon-P PVDF Membrane 10 sheets, 15 cm x 15 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
IPVH20200 Immobilon-P PVDF Membrane 10 sheets, 20 cm x 20 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
IPVH304F0 Immobilon-P PVDF Membrane 10 sheets, 26 cm x 26 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
ISEQ00005 Immobilon-PSQ PVDF Membrane 1 roll, 26.5 cm x 1.875 m, 0.2 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
ISEQ00010 Immobilon-PSQ PVDF Membrane 1 roll, 26.5 cm x 3.75 m, 0.2 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
ISEQ07850 Immobilon-PSQ PVDF Membrane 50 sheets, 7 cm x 8.4 cm, 0.2 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
ISEQ08100 Immobilon-PSQ PVDF Membrane 10 sheets, 8 cm x 10 cm, 0.2 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
ISEQ08130 Immobilon-PSQ PVDF Membrane 10 sheets, 8.5 cm x 13.5 cm, 0.2 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
ISEQ09120 Immobilon-PSQ PVDF Membrane 10 sheets, 9 cm x 12 cm, 0.2 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
ISEQ10100 Immobilon-PSQ PVDF Membrane 10 sheets, 10 cm x 10 cm, 0.2 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
ISEQ15150 Immobilon-PSQ PVDF Membrane 10 sheets, 15 cm x 15 cm, 0.2 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
ISEQ20200 Immobilon-PSQ PVDF Membrane 10 sheets, 20 cm x 20 cm, 0.2 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
ISEQ26260 Immobilon-PSQ PVDF Membrane 10 sheets, 26 cm x 26 cm, 0.2 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
WBKLS Immobilon Western Chemiluminescent HRP Substrate Western blotting, dot/slot blotting
N6645 Immobilon®-NC HAHY membranes L × W 10 ft × 1 ft
P2438 Immobilon®-P Polyvinylidene difluoride membranes size 9 cm × 12 cm
P2688 Immobilon®-P Polyvinylidene difluoride membranes size 20 cm × 20 cm
P2813 Immobilon®-P Polyvinylidene difluoride membranes size 26 cm × 26 cm