HPLC Analysis of Glycans

The glycan pool released from a glycoprotein can be separated into components depending upon the characteristics of the glycans and the HPLC matrices used. Normal phase, weak anion exchange, and reversed phase chromatography are most commonly used in conjunction with fluorescentlabeled oligosaccharides for the separation of sialylated and neutral N- and O-linked glycans. High pH anion exchange chromatography with pulsed amperometric detection (HPAE-PAD) can be used with native glycans, but it has a major limitation in that the separation is achieved in high molarity sodium hydroxide that must be removed prior to recovering glycans for further structural analysis.
For more information on HPLC Analysis of Glycans please browse our online Glycobiology Analysis Manual.

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D3818 Dextran Ladder Glycan standard for HPLC