Matricies for Mass Spectroscopy Analysis of Glycans

The development of modern mass spectrometry (MS) equipment with high resolution and mass accuracy has led to its use in analyzing glycans for both profiling and structural studies. Unlike the HPLC method, a substantially larger amount (~10-20 times) of glycan is normally required for a single MS spectrum. Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry is the most widely used MS technique. The information gained by using MALDI MS is mass weight, which can be used to assign putative monosaccharide structures present in a pure oligosaccharide since the mass of a monosaccharide is measured with a high degree of accuracy. MALDI MS can be used in conjunction with glycan sequencing using exoglycosidase enzymes for structural and linkage analysis of glycans.

Different approaches and matrices are used for the analysis of neutral and acidic glycans (those that contain sialic acid) by MALDI MS. Neutral glycans ionize with reasonable efficiency using 2,5-dihydroxybenzoic acid (2,5-DHB) as the matrix in positive ion mode. Acidic glycans generally provide poor MALDI spectra with DHB matrices due to variable losses of sialic acids or carboxyl groups, leading to multiple peaks. These glycans are therefore analyzed in negative ion mode using an alternative matrix as described later.

For more information on Mass Spectrometry of Glycans please browse our online Glycobiology Analysis Manual.

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08658 8-Aminonaphthalene-1,3,6-trisulfonic acid disodium salt BioReagent, suitable for fluorescence, ≥90% (CE)
A77989 2-Aminopyridine ≥99%
A77997 2-Aminopyridine 99%
09340 2-Aminopyridine purum, ≥98.0% (NT)
A89804 Anthranilamide ≥98%
A89855 Anthranilic acid reagent grade, ≥98%
10680 Anthranilic acid puriss. p.a., ≥99.5% (T)
125571 5-Chloro-2-mercaptobenzothiazole technical grade, ≥90%
C8982 α-Cyano-4-hydroxycinnamic acid suitable for MALDI-TOF MS
37468 2′,6′-Dihydroxyacetophenone matrix substance for MALDI-MS, ≥99.5%
85707 2,5-Dihydroxybenzoic acid matrix substance for MALDI-MS, >99.0% (HPLC)
103276 Harmane 98%
N6252 Norharmane crystalline
85590 Spermine ≥99.0% (GC)
91928 2′,4′,6′-Trihydroxyacetophenone monohydrate matrix substance for MALDI-MS, ≥99.5%