Reagents

Reagents are substances used to detect the presence of other substances; hence, they are critical components of many laboratory procedures. Sigma-Aldrich provides an extensive list of Molecular Biology grade reagents used to purify plant nucleic acid in cetrifugation, phase separation, precipitation, and other DNA and RNA isolation and extraction procedures. GenElutekits include many of the necessary reagents. Reagents that can be purchased separately include buffers, detergents, solvents, and inhibitors, in powder, pellet, and solution forms.

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B7906 1-Butanol for molecular biology, ≥99%
C3032 Cesium chloride for molecular biology, ≥99% (silver nitrate titration)
C4036 Cesium chloride for molecular biology, ≥98%
C2432 Chloroform contains 100-200 ppm amylenes as stabilizer, ≥99.5%
C0549 Chloroform:Isoamyl alcohol 24:1 suitable for nucleic acid purification
E4274 Endotoxin Removal solution Remove lipopolysaccharides (LPS) from plasmid DNA preparations
G1767 Glycogen from Mytilus edulis (Blue mussel) for DNA precipitations
G3272 Guanidine hydrochloride for molecular biology, ≥99%
G9277 Guanidine thiocyanate for molecular biology, ≥99%
H6269 Hexadecyltrimethylammonium bromide for molecular biology, ≥99%
L9650 Lithium chloride for molecular biology, ≥99%
L7026 Lithium chloride solution 8 M, for molecular biology, ≥99%
I9392 3-Methylbutanol BioReagent, for molecular biology, ≥98.5%
P1037 Phenol for molecular biology
P4557 Phenol solution BioReagent, Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, for molecular biology
P4682 Phenol solution BioReagent, Saturated with 0.1 M citrate buffer, pH 4.3 ± 0.2, for molecular biology
P1944 Phenol:Chloroform 5:1 for molecular biology
P3803 Phenol:Chloroform:Isoamyl Alcohol 25:24:1, Saturated with 10mM Tris, pH 8.0, 1mM EDTA for molecular biology
P5413 Poly(ethylene glycol) for molecular biology, average mol wt 8,000
P5288 Polyvinylpyrrolidone for molecular biology, nucleic acid hybridization tested, mol wt 360,000
P1190 Potassium acetate for molecular biology, ≥99.0%
I9516 2-Propanol BioReagent, for molecular biology, ≥99.5%
S2889 Sodium acetate anhydrous, for molecular biology, ≥99%
S7899 Sodium acetate buffer solution pH 5.2±0.1 (25 °C), for molecular biology, 3 M, 0.2 μm filtered
S2404 Sodium acetate buffer solution BioXtra, pH 7.0±0.05 (25 °C), for molecular biology, 3 M, non-sterile; 0.2 μm filtered
S8045 Sodium hydroxide BioXtra, ≥98% (acidimetric), pellets (anhydrous)
S5881 Sodium hydroxide reagent grade, ≥98%, pellets (anhydrous)
S1141 Spermine tetrahydrochloride BioReagent, for molecular biology
W4502 Water Molecular Biology Reagent