Enzymatic Deglycosylation

Sequential hydrolysis of individual monosaccharides from glycans can be useful for the elucidation of the structure and function of the glycan component. Due to the restraints of the specificity of glycolytic enzymes currently available, sequential hydrolysis of individual monosaccharides is also required in many instances in order to completely remove a glycan component enzymatically. This is particularly true in the enzymatic deglycosylation of many O-linked glycans. Both endoglycosidases and exoglycosidases are used for deglycosylation procedures. Endoglycosidases are enzymes that catalyze the cleavage of an internal glycoside bond in an oligosaccharide. Exoglycosidases are enzymes that remove terminal carbohydrates from the non-reducing end of a glycan, but do not cleave internal bonds between carbohydrates.
For more information on Glycobiology please browse our online Glycobiology Analysis Manual.