Gelling Agents

Plant tissue culture takes place in three-dimensional space. Callus cells, singly or in very small clusters, can be grown in liquid media, but for shoots to extend up and then roots to grow down, the callus cells are plated on a semi-solid gel surface. We offer a variety of gelling agents to allow the researcher to optimize the regeneration conditions for each species. The gelling agent of choice may change with the stage of the plant development. Agar is often the first choice to solidify the nutrient culture medium. It is a natural polysaccharide product extracted from red algae seaweed.. We offer agar choices that differ in degree of purity as well as gelling temperature, trace element composition, ash content, and pH. Agarose, the gelling component of agar, may be used in suspension cultures as well as gels. PhytagelTM ,our bacterially produced polysaccharide composed of glucuronic acid, rhamnose, and glucose, is a superior replacement for agar in some cases. Phytagel is clear, colorless, and makes a high strength gel. AgargelTM blends Phytagel with agar. Alginic acid, extracted from brown algae, is used to encapsulate protoplasts.

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A1296 Agar microbiology tested, plant cell culture tested, cell culture tested, powder
A7921 Agar plant cell culture tested, powder
A4550 Agar Type A, plant cell culture tested
A9799 Agar High gel strength, plant cell culture tested
A6686 Agar Bacteriological, microbiology tested, plant cell culture tested, granular
A4675 Agar Type E, plant cell culture tested
A8678 Agar plant cell culture tested
A4800 Agar Type M, plant cell culture tested
A3301 Agargel suitable for plant cell culture, powder
A0682 Alginic acid sodium salt from brown algae BioReagent, plant cell culture tested, low viscosity, powder
G1910 Gelzan CM Gelrite®
P8169 Phytagel BioReagent, plant cell culture tested, powder