Gene Expression Analysis

Gene expression involves synthesis of a functional gene product (protein, enzyme or RNA) from DNA, by first transcribing it into mRNA, then splicing, translation, and finally post-translational modification. Product analysis probes evolutionary change, since the timing, location, and quantity of gene expression affects cell functions, and the geneotype affects the phenotype. Genomic profiling technologies can quantify various biological subsystems (epigenome, transcriptome, phosphorylome, metabolome) through biochemistry, molecular genetics, structural biology, cell biology and imaging.

Random primers with single-stranded DNA templates produce initiation sites for DNA polymerases, and labeled probes can be complementary to the entire template sequence. Random primers (hexanucleotide, hexanucleotides, hexamers, nonamers, 9-mers, Oligo(dT)23 Primers (23 thymidine residues and one 3’ G, C, or A residue) can make radioactive or non-radioactive probes using a DNA polymerase and a DNA template. They may be used as universal primers in first strand cDNA synthesis (from poly(A)+ RNA, for example), cDNA library construction, RT-PCR and other applications.

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A0410 Aminoallyl-dUTP sodium salt ≥85%, lyophilized powder
A5660 Aminoallyl-UTP sodium salt ≥75%, lyophilized powder
H0268 Hexanucleotide Primers lyophilized powder
O4387 Oligo(dT)23, Anchored 70 μM in H2O
R7647 Random Nonamers for use as primers in cDNA synthesis, 50 μM in H2O