Directional Cloning System

Directional cloning is a highly efficient method of directing the orientation of clonal inserts into vectors. By digesting a DNA insert or vector molecule with two restriction endonuclease enzymes, the researcher can create either blunt or non-complementary sticky ends at both ends of each restriction fragment using restriction digestion, PCR (polymerase chain reaction), or other physical or enzymatic methods. The insert can then be ligated to the vector (plasmid or bacteriophage) in a specific, fixed orientation, without the risk of self-ligation by the cohesive ends during the cohesion. Protein-coding regions of a gene can be transferred, without non-coding or non-interesting regions.

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RDC1 Director Universal PCR System
LIG2 QuickLink DNA Ligation Kit joins blunt-end and sticky-end DNA fragments