Additional Affinity Purification

Each recombinant protein expressed has its own requirements for optimal conditions to maintain structure and function. Similarly, in the production of fusion proteins, different peptide tags may be optimal; suitability may depend upon the plant producing the recombinant, folding of the expressed target protein with a specific tag, and sensitivity of the target protein to conditions used in affinity purification. We offer immunoaffinity gels specific for the short peptides c-myc, V5, and residues 98-106 of human influenza virus hemagluttinin (HA-tag) as well as c-myc,V5 and HA-tag peptides for elution. All can be used in plant expression systems. Thioredoxin, 108 amino acids, has been used in fusion proteins; we supply a polyclonal antibody-agarose to purify thioredoxin tagged proteins. EZview Red affinity gel coupled to Protein A or Protein G can increase the precision of small-scale immunoprecipitation or antibody capture assays.

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M2435 c-Myc Peptide lyophilized powder
P6486 EZview Red Protein A Affinity Gel
E3403 EZview Red Protein G Affinity Gel
A2095 Monoclonal Anti-HA−Agarose antibody produced in mouse clone HA-7, purified immunoglobulin, PBS suspension
F5551 Hemagglutinin, Filamentous from Bordetella pertussis lyophilized powder
GE29-0485-86 HisTrap Excel GE Healthcare, 29-0485-86, pack of 1 mL
GE17-5255-01 HisTrap Fast Flow GE Healthcare, 17-5255-01, pack of 5 × 5 mL
GE17-5286-01 HisTrap Fast Flow Crude GE Healthcare, 17-5286-01, pack of 5 × 5 mL
GE29-0486-31 HisTrap Fast Flow Crude GE Healthcare, 29-0486-31, pack of 1 mL
GE17-5247-01 HisTrap High Performance GE Healthcare, 17-5247-01, pack of 5 × 1 mL
GE17-5248-02 HisTrap High Performance GE Healthcare, 17-5248-02, pack of 5 × 5 mL
GE29-0510-21 HisTrap High Performance GE Healthcare, 29-0510-21, pack of 1 mL
I2149 Influenza Hemagglutinin (HA) Peptide ≥97% (HPLC)
A7470 Anti-c-Myc Agarose Affinity Gel antibody produced in rabbit affinity isolated antibody
A2582 Anti-Thioredoxin–Agarose antibody produced in rabbit IgG fraction of antiserum, PBS suspension
A7345 Anti-V5 Agarose Affinity Gel antibody produced in mouse purified immunoglobulin, clone V5-10
V7754 V5 Peptide ≥97% (HPLC), lyophilized powder