Protease Inhibitors

Sigma-Aldrich offers a wide range of protease inhibitors to ensure that proteolytic degradation does not occur during plant cell lysis and protein extraction.

Protease inhibitors are molecules that inhibit the function of a large group of naturally occurring enzymes called proteases. Protease inhibitors prevent proteases from splitting proteins into peptides. Proteases can either break specific peptide bonds or break down a complete peptide to amino acids (unlimited proteolysis).

During plant protein isolation procedures, proteases can quickly degrade proteins and drastically reduce their yield for purification. Sigma-Aldrich’s protease inhibitor cocktails have been optimized to maintain and preserve protein functionality following cell lysis and are available in powder, DMSO solution, or tablet form. They are proprietary blends of protease inhibitors specific for plant cells. SIGMAFAST protease inhibitor cocktail tablets are EDTA-free for use in purification of metal-dependent and Histidine-tagged proteins. Actions include the inhibition of serine, cysteine, aspartic, metalloproteases, and aminopeptidases.

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P9599 Protease Inhibitor Cocktail for plant cell and tissue extracts, DMSO solution
P2714 Protease Inhibitor Cocktail powder for general use, lyophilized powder
P8465 Protease Inhibitor Cocktail powder for use with bacterial cell extracts, lyophilized powder
S8830 SIGMAFAST Protease Inhibitor Cocktail Tablets, EDTA-Free for use in purification of Histidine-tagged proteins