Nucleic Acid Hybridization Reagents

Blocking Reagents, Buffers, & Reagents for DNA Hybridization

DNA hybridization relies on fundamental DNA base pairing through hydrogen bonding. DNA molecules bind most strongly to the DNA molecule that has the closest match to its sequence (complementary sequence). Hybridization conditions can be optimized so one sequence will only bind to its exact partner sequence. This allows identification of the presence of a DNA sequence using a different DNA sequence.

The probe (usually labeled with P32, fluorescent dyes, or biotin) is a known sequence. If it binds to the sample, then it is assumed that the target is present. DNA hybridization is used in techniques such as Southern blotting, chromosome painting, probe-based qPCR, dot blots, and arrays. A clear hybridization result relies on a combination of factors. One such factor is non-specific hybridization of the probe to a substrate. To avoid this effect, blocking reagents with sonicated and denatured DNA can be added.

Get additional information on the specific mechanisms of blocking reagents, buffers, and DNA hybridization products on individual product pages.