Gel Casting Reagents

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01703 Acrylamide : N,N′-Methylenebisacrylamide 32.5:1 for electrophoresis  
A7802 Acrylamide/Bis-acrylamide, 40% solution BioReagent, suitable for electrophoresis, 29:1 These acrylamide/bis-acrylamide solutions are available at the most common ratios for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.
01704 Acrylamide:N,N′-Methylenebisacrylamide 24:1 for electrophoresis  
01708 Acrylamide:N,N′-Methylenebisacrylamide 29:1 solution for electrophoresis, mixture of acrylamide and BIS 29:1, 40% in H2O  
01702 Acrylamide:N,N′-Methylenebisacrylamide 29:1 BioXtra, for molecular biology  
01709 Acrylamide:N,N′-Methylenebisacrylamide 37.5:1 solution for electrophoresis, mixture of acrylamide and BIS 37.5:1, 40% in H2O  
GE17-0468-01 Agarose IEF GE Healthcare, 17-0468-01, pack of 10 g  
E0389 Electrophoresis film for polyacrylamide gels 7 mil (0.2 mm) thickness Electrophoresis film is a flexible, transparent polyester film specifically designed to support polyacrylamide gels. Ideal for casting horizontal IEF gels. One side has been treated with a hydrophilic coating which enhances adhesion with aqueous polyacrylamide gel media. The acrylamide monomers covalently attach to the coating during polymerization. Gels remain attached during electrophoresis, staining and drying.
56741 IGEPAL® CA-630 IGEPAL® CA-630 has been used:
• In phosducin co-precipitation assay for pull down of Epitope-Tagged HAGγ1 and HAGγ1C71S
• As a component of lysis buffer for cell culture and sample preparation for MAPK (mitogen-activated protein kinases) analysis, Immunoblotting
• In the extraction of cells in Oil Red O staining procedure to visualise lipid formation in cells during adipogenesis
• As a component of lysis buffer containing Tris-HCl, NaCl and EDTA for immunoblotting experiment

U6504 Urea for electrophoresis A chaotropic agent used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis.
Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.