Quantitation Kits and Reagents

Protein Determination is one of the most common operations performed in biochemical research. The principle of the bicinchoninic acid (BCA) assay is similar to the Lowry procedure, in that both rely on the formation of a Cu2+-protein complex under alkaline conditions, followed by reduction of the Cu2+ to Cu1+. The amount of reduction is proportional to the protein present. It has been shown that cysteine, cystine, tryptophan, tyrosine, and the peptide bond are able to reduce Cu2+ to Cu1+. BCA forms a purple-blue complex with Cu1+ in alkaline environments, thus providing a basis to monitor the reduction of alkaline Cu2+ by proteins at absorbance maximum 562 nm. The BCA assay is more sensitive and applicable than either Biuret or Lowry procedures. In addition, it has less variability than the Bradford assay. The BCA assay has many advantages over other protein determination techniques:

  • The color complex is stable.
  • There is less susceptibility to detergents.
  • It is applicable over a broad range of protein concentrations.

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D8284 Bicinchoninic acid disodium salt hydrate ≥98% (HPLC)
B9643 Bicinchoninic Acid solution
BCA1 Bicinchoninic Acid Kit for Protein Determination for 200-1000 μg/ml protein
BPQ96 BioPORTER® QuikEase Protein Delivery Kit
B3934 Biuret reagent protein detection level 150-1,000 μg/mL
B6916 Bradford Reagent for 0.1-1.4 mg/ml protein
GE17-0445-01 Calibration Kit High Molecular Weight For Electrophoresis GE Healthcare, 17-0445-01, pkg of 10 vials
GE17-0446-01 Calibration Kit Low Molecular Weight For Electrophoresis GE Healthcare, 17-0446-01
GE80-6484-51 2-D Clean-Up Kit GE Healthcare, 80-6484-51
C2284 Copper(II) sulfate solution 4 % (w/v) (prepared from copper (II) sulfate pentahydrate)
F9015 Fluorescamine ≥98% (TLC), powder, used for detection of primary amines
F9252 Folin & Ciocalteu’s phenol reagent suitable for determination of total protein by Lowry method, 2 N
L3540 Lowry Reagent
P2297 Picrylsulfonic acid solution BioReagent, 5 % (w/v) in H2O, suitable for determination of primary amines
51254 Protein Quantification Kit-Rapid sufficient for 500 tests
QPBCA QuantiPro BCA Assay Kit for 0.5-30 μg/ml protein
GE80-6483-56 2-D Quant Kit GE Healthcare, 80-6483-56
TP0300 Total Protein Kit, Micro Lowry, Peterson’s Modification