KiCqStart® qPCR master mix reagents — formatted for your instrument

Quantitative PCR (qPCR) uses the linearity of DNA amplification to determine absolute or relative amounts of a known sequence in a sample. By using a fluorescent reporter in the reaction, it is possible to measure DNA generation in real time.

In qPCR, DNA amplification is monitored at each cycle of PCR. When the DNA is in the log linear phase of amplification, the amount of fluorescence increases above the background. The point at which the fluorescence becomes measurable is called the Threshold cycle (CT) or crossing point. By using multiple dilutions of a known amount of standard DNA, a standard curve can be generated of log concentration against CT. The amount of DNA or cDNA in an unknown sample can then be calculated from its CT value.  

The KiCqStart® qPCR reagents are rigorous ready mixes that are supplied formatted for specific thermo cylclers. These qPCR reagents are formulated to be easily adaptable to your specific application and needs.  

Specific and linear qPCR results

  • Stringent hot-start mechanism for greater specificity
  • Modified enzyme for cDNA synthesis at temperature up to 55 °C
  • More tolerant of PCR inhibitors in samples
  • Linear up to 6 logs of input template

 

Linear Dynamic Range

Flexible, easy-to-use qPCR mixes

  • Ready mixes with all non-custom reaction components
  • Design your own detection from a variety of probe chemistries
  • Run experiments in conventional or fast PCR mode
  • Formats available for all major qPCR instruments

 

Hemoglobin Tolerance

KiCqStart® Probe qPCR ReadyMix reagent provides sensitivity across a range of template input amounts. 

 

KiCqStart® One-Step Probe RT-qPCR ReadyMix reagent is highly resistant to common sample-based inhibitors. 

 

Basic Process for using qPCR Master Mixes

KiCqStart® ReadyMix reagents are ready-to-use, highly sensitive master mixes containing all the basic components for qPCR or RT-qPCR. Simply add your primers, probe and water to complete the assay cocktail. Add the cocktail to tubes or plates and then add your template. The KiCqStart® ReadyMix™ reagents are compatible with conventional or fast PCR mode. For more details, see product usage information at KiCqStart® Probe qPCR ReadyMix or KiCqStart® One-Step Probe RT-qPCR ReadyMix™ reagents.

 

Design Your qPCR Detection Method

KiCqStart® reagents are compatible with a variety of fluorescent probe chemistries, including TaqMan® Probes (also called hydrolysis probes or dual-labeled probes), molecular beacons, LIghtCycler® Probes, and Scorpions® Probes. Select from options below to learn more.

Dual-labeled Probes
Molecular Beacons
   
LightCycler Probes
Scorpions Probes

 

qPCR Reagents Formatted for Your Instrument

Different real-time PCR systems use different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument. KiCqStart® ReadyMix™ reagents contain ROX passive reference dye at concentrations recommended by these (and other) popular cyclers.

Real-time PCR Instrument Name KiCqStart® Probe qPCR ReadyMix™

KiCqStart® One-Step Probe RT-qPCR ReadyMix™
KiCqStart® Probe qPCR ReadyMix™, Low ROX™

KiCqStart® One-Step Probe RT-qPCR ReadyMix™, Low ROX™
KiCqStart® Probe qPCR ReadyMix™, ROX™

KiCqStart® One-Step Probe RT-qPCR ReadyMix™, ROX™
  KCQS04; KCQS07 KCQS05; KCQS08 KCQS06; KCQS09
Agilent AriaMX    
Applied Biosystems 5700    
Applied Biosystems 7000    
Applied Biosystems 7300    
Applied Biosystems 7500    
Applied Biosystems 7500 Fast    
Applied Biosystems 7700    
Applied Biosystems 7900    
Applied Biosystems 7900 HT    
Applied Biosystems 7900 HT Fast    
Applied Biosystems StepOnePlus™    
Applied Biosystems StepOne™    
Applied Biosystems ViiA™ 7    
Applied Biosystems QuantStudio™    
BioRad CFX    
Bio-Rad CFX384™    
Bio-Rad® CFX96™    
BioRad iCycler iQ™      
BioRad iQ™5      
Bio-Rad MiniOpticon™    
BioRad MyiQ™      
Bio-Rad/MJ Chromo4™    
Bio-Rad/MJ Opticon 2™    
Bio-Rad/MJ Opticon™    
Cepheid SmartCycler®    
Douglas Scientific IntelliQube®    
Eppendorf Mastercycler® ep realplex    
Eppendorf Mastercycler® ep realplex2 S    
Illumina Eco™ qPCR    
Qiagen/Corbett Rotor-Gene® 3000    
Qiagen/Corbett Rotor-Gene® 6000    
Qiagen/Corbett Rotor-Gene® Q    
QIAGEN Rotor-Gene Q    
Roche LightCycler™ 480    
Stratagene Mx3000P™    
Stratagene Mx3005P™    
Stratagene Mx4000™    

 

Products

Detailed KiCqStart® product information is listed below. To learn more about qPCR, see our PCR Technologies Guide or qPCR and MIQE Seminar Series.
 

Product No. Description
KCQS00 KiCqStart SYBR® Green qPCR ReadyMix For Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems
KCQS01 KiCqStart SYBR® Green qPCR ReadyMix Low ROX™, for ABI and Stratagene instruments
KCQS02 KiCqStart SYBR® Green qPCR ReadyMix with ROX™ for ABI instruments
KCQS03 KiCqStart SYBR® Green qPCR ReadyMix iQ™, with fluorescein for Bio-Rad systems
KCQS04 KiCqStart Probe qPCR ReadyMix for Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems
KCQS05 KiCqStart Probe qPCR ReadyMix, Low ROX™ with Low ROX for ABI and Stratagene instruments
KCQS06 KiCqStart Probe qPCR ReadyMix, ROX™ with ROX for ABI instruments
KCQS07 KiCqStart One-Step Probe RT-qPCR ReadyMix for Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems
KCQS08 KiCqStart One-Step Probe RT-qPCR ReadyMix, Low ROX™ with Low ROX for ABI and Stratagene instruments
KCQS09 KiCqStart One-Step Probe RT-qPCR ReadyMix, ROX™ with ROX for ABI instruments