Superior Amplification Efficiency with Lower CT

Quantitative PCR was performed on human genomic DNA. The template was diluted 10-fold in subsequent wells. A TaqMan® probe and primers specific for a 250 bp PCR product of the ß-actin gene were used with JumpStart Taq ReadyMix for Quantitative PCR (D7440) or a master mix from Competitor A. The JumpStart Taq ReadyMix (in red) has better amplification efficiency (Figure 1), resulting in lower CT values than Competitor A (in blue) (Figure 2).