CT Values for Lambda Amplicon using SYBR Green JumpStart Taq ReadyMix

SYBR Green JumpStart Taq ReadyMix (S4438) was added to dilutions of pBAC–2cp template and plasmid specific primers. Initial template copy number was 106 and and diluted 10–fold in subsequent wells. 10–1 signifies a 10–fold dilution of the previous well. Specific primers were designed to produce a 311 base pair amplicon. Reactions were carried out and analysis was done on the ABI Prism® 7700 Sequence Detection System.