Bacterial Cell Lysis

BugBuster® Protein Extraction Reagent is formulated to gently disrupt the cell wall of E.coli and liberate soluble proteins. This flagship reagent from MilliporeSigma utilizes a detergent mix that is capable of cell wall perforation without denaturing soluble protein. Simply add the BugBuster® Reagent to your cell pellet and incubate at room temperature for 10 minutes! No special preparation of your samples is required. No instruments are needed. Unlike other mechanical methods such as Sonication, protein denaturation is not a problem. BugBuster® reagent produces high yields that are reproducible, convenient and fast!

Product Advantages:

  • Standard reagent is supplied as a Tris-buffered "1X" ready-to-use liquid
  • Stable at room temperature
  • Performance can be enhanced using Benzonase® nuclease and rLysozyme™ solution

BugBuster® Master Mix combines BugBuster® Protein Extraction Reagent with Benzonase® Nuclease and rLysozyme Solution in one convenient reagent.

Featured Application Note

Simplifying the Bacterial Recombinant Protein Workflow while Maximizing Recovery

A Complete Solution from Expression to Detection

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BugBuster® reagent is superior to "Home Brew" lysis buffer and BugBuster® reagent with both Benzonase® nuclease and rLysozyme solution yielded lysates with the most 6XHis-CRP. (A) E.coli lysates (5μL of 1 mL total lysate) from various lysis protocols were fractionated and analyzed by SDS-PAGE. A band correspondinf to 6XHIS-CRP is prominently visualized in the BB +/+ lane.

Components of Bacterial Lysis Reagents
  BugBuster® Buffer Benzonase® rLysozyme™ Notes
BugBuster® X X      
BugBuster® 10X X       Flexibility to customize dilution & buffer composition
BugBuster® Plus Benzonase® X X X   2 separate vials for greater flexibility
BugBuster® Plus Lysonase™ X X X X 2 separate vials for greater flexibility
BugBuster® Master Mix X X X X 1 convenient reagent
PopCulture® X X     Buffer protects protein from the pH extremes produced in high density culture