Mammalian Cell Lysis

Choose a mammalian cell lysis reagent based on your sample and downstream detection methods. MilliporeSigma’s lysis reagents support preparation of mammalian and insect proteins, including cytosolic, nuclear and phosphorylated proteins.

CytoBuster™ Protein Extraction Reagent is a proprietary formulation of detergents optimized for fast, efficient extraction of soluble proteins from mammalian and insect cells to be used in activity assays.

The PhosphoSafe™ Extraction Reagent is a detergent and phosphatase inhibitor mixture optimized for fast, efficient extraction of soluble proteins from mammalian and insect cells and preserves the phosphorylation state of extracted proteins.

ProteoExtract Kits – dedicated extraction kits from mammalian cells and tissues.

Research Poster:

Optimizing Extraction of β-amyloid from Brain Tissue

 
 
Click image to enlarge.

In Direct Extraction, CytoBuster™ reagent (“Cyto”), TBS and Triton® X-114 appeared to be more efficient in solubilizing Aβ 1-40 fragment than Formic Acid (FA).

Presented at Neuroscience 2015 Conference

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