Integrated Solutions for Mass Spectrometry

Minitip for MS Analysis

Superior Recovery, Exceptional Binding

Expertly designed by chromatographers and mass spectrometrists, the MiniTip C18 pipette tip provides superior sample recovery, increased bed integrity, and enhanced binding capacity for purifying and concentrating mass spectrometry samples. MiniTips’ unique, proprietary adhesive chemistry greatly increases available silica particle surface area, leading to exceptional binding capacity and unsurpassed analyte recovery. The unique solid phase support binds peptides, proteins, and other analytes retained by reversed phase techniques while withstanding the rigors of repeated draw and dispense cycles without structural degradation. For optimal purification and concentration of important samples, trust MiniTips!

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Order Information

Product Code Product Name Size
TPSC18 MiniTip C18   96 tips
  960 tips

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Superior Recovery

Mass spectrometrists are often concerned that important samples may be lost during concentration and purification. To address this concern, Sigma MiniTips have been designed to provide the most efficient recovery available.

MiniTips' proprietary adhesive chemistry and optimum adhesive to silica ratio maximize the available silica surface area, which is nearly three times that of competitors. This increased surface area enables greater C18 binding and greater affinity for protein and peptides samples. During repeated draw and dispense cycles, more sample is retained by the MiniTip, and more sample is subsequently recovered.

More Efficient Recovery, Greater Reproducibility
Peptide Signal After Purification from 400 µM Urea

In an experiment evaluating MiniTips and two competitors, peptide samples were purified, eluted and analyzed by MALDI-MS. Signals for the samples prepared using MiniTips were greatly enhanced when compared to competing tips. MiniTips also perform more consistently than alternative tips. In the experiment described above, MiniTips exhibited an average relative standard deviation of 14%. Competitor M's average relative standard deviation was 73% and Competitor E's average relative standard deviation was 94 %.

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Enhanced Affinity, Exceptional Binding Capacity

Sigma MiniTips consistently demonstrate both improved analyte affinity and superior binding capacity when compared to other tips.

MiniTips' greater silica surface area leads to additional C18 binding and increases affinity for proteins and peptides. As analytes are drawn through the C18 silica, they are retained more readily and in greater quantities than in competitors' tips. Enhanced affinity ensures that even low abundance samples are retained and purified.

MiniTips saturate with analyte at a much higher concentration than other tips, enabling purification of larger samples. Increased binding capacity is particularly beneficial when analysis conditions require more sample. In an experiment using ß-amyloid, MiniTips demonstrated nearly twice the binding capacity of an alternative tip.

ß-Amyloid Binding Capacity (µg)

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Stable Bed Minimizes Degradation and Contamination

Competitor M after use.

MiniTip after use.

Even under the most demanding conditions, the design of the MiniTip prevents sorbent bed degradation. While other tips collapse under standard conditions, leaving contaminating material in valuable samples, the MiniTip maintains its integrity.

A Sigma MiniTip and a competitor's C18 tip were used to purify and concentrate a protein sample. Upon coloration of the silica within the tips, it was evident that competitor M's sorbent bed was broken and fragmented while that of the MiniTip remained intact, demonstrating its integrity.

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Improved Data Quality from Clean-up

Without sample cleanup, data acquisition can be difficult if not futile. Concentrating and purifying peptides with a C18 MiniTip enables signal enhancement and reliable protein identification. Typical sample cleanup is performed from solutions containing denaturants, chaotropes, and buffer salts. MiniTips can be used to effectively bind low abundance and low copy proteins and concentrate them by eluting in a small volume. In some cases, peptide mass fingerprint samples can be eluted in as little as 1 µL of matrix for immediate sample spotting.


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