Bacterial Cell Lysis

CelLytic IB

CelLytic IB was designed to solubilize protein aggregates called inclusion bodies (Figure 1). In bacteria, inclusion bodies are sometimes formed when recombinant proteins are overexpressed. CelLytic IB was formulated to solubilize the protein of interest for immediate analysis of protein content or refolding procedures.

Product #  Product Name Package Size
C5236 CelLytic IB Cell Lysis Reagent 25 ml
100 ml

Figure 1. Solubilization of Streptavidin Inclusion Bodies.
Samples of solubilized inclusion body protein were assayed using BCA Reagent (BCA-1). 50 µl of each sample was incubated with BCA reagent at 60 °C for 15 min. The samples were then cooled to room temperature and assayed at 562 nm. The data above has been standardized to the protein recovery of CelLytic IB.

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