Mammalian Cell Lysis

CelLytic Nu-CLEAR

Within this kit is a complete system for preparing nuclear and cytoplasmic protein extracts from mammalian tissue or cultured cell. A number of different procedures in the detailed technical bulletin enable the selection that best fits a particular application. For example, choose between detergent and non-detergent extraction of nuclear protein or between the standard hypotonic lysis buffer for most cell types and isotonic lysis buffer for fragile cells. In addition, the kit provides a procedure for salt reduction from the nuclear extract with dilution buffer. CelLytic Nu-CLEAR offers the flexibility you need for optimal protein extraction. Extracts can be prepared in less than 2 hours and are highly pure since there is little or no cross-contamination between nuclear and cytoplasmic extracts (Figure 1). Each kit is sufficient for 100 extractions of 100 µl of packed cell volume.

Product #  Product Name Package Size
N-XTRACT CelLytic Nu-CLEAR Extraction Kit 1 kit

Figure 1. Purity of Cytoplasmic and Nuclear Proteins.
The double stranded 32P-labeled Octamer motif oligonucleotide was incubated with either cytoplasmic fraction (C) or nuclear extract (N) prepared from HeLa, CHO, COS and PC-12 cells using the CelLytic Nu-CLEAR extraction kit.

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