Proteomics & Protien Expression

Preparing GST Fusion Proteins

Preparing GST Fusion Proteins We have prepared product lists for commonly used protocols from Cold Spring Harbor Protocols.

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Selected Protocol from "Protein-Protein Interactions", 2nd Edition, E. Golemis, P. Adam.

Preparation of Glutathione-S-Transferase (GST) Fusion Proteins

Margret B. Einarson and Elena N. Pugacheva
Foxx Chase Cancer Center, Philadelphia, PA 19111

Jason R. Orlinck
Brigham & Women’s Hospital, Boston, MA 02115


Glutathione-S-Transferase (GST) fusion proteins have a wide range of applications. This protocol is designed for IPTG-inducible bacterial expression vectors.  

Products Available for this Protocol
Protocol Material Description Product # Product Name Add to Cart
Reduced glutathione, 20 mM prepared in 50 mM Tris-HCl (pH 8.0) G4251 L-Glutathione reduced
Isopropyl-β-D-thio-galactoside (IPTG) I5502 Isopropyl β-D-1-thiogalactopyranoside
LB broth, containing appropriate antibiotic selection L2542 LB Broth
PBS P5368 Phosphate buffered saline
1% Triton X-100 X-100 Triton® X-100
Aprotinin A1153 Aprotinin from bovine lung
Leupeptin L9783 Leupeptin hydrochloride
Phenylmethylsulfonyl fluoride [PMSF] P7626 Phenylmethanesulfonyl fluoride
150 mM NaCl S3014 Sodium chloride
20 mM sodium phosphate (pH 7.4) S3264 Sodium phosphate dibasic
Glutathione-Sepharose beads G4510 Glutathione−Agarose
Coomassie blue (see Sambrook and Russell 2001) B5133 Brilliant Blue G
100 mM Tris-HCl (pH 6.8) T3253 Trizma® hydrochloride
4%(w/v) SDS (electrophoresis grade) L4390 Sodium dodecyl sulfate
0.2% (w/v) bromophenol blue B0126 Bromophenol Blue
20% (v/v) glycerol G9012 Glycerol
200 mM dithiothreitol (DTT) D9779 DL-Dithiothreitol
β-mercaptoethanol M3148 2-Mercaptoethanol


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Product Association Disclaimer: The products listed for this specific protocol were selected either to match or to supplement the products listed within the actual protocol. The products/reagents have been qualified for usage, but may not have been validated for this specific application. Please refer to the detailed product description on the usage of specific products of interest.