Purification & Detection

FLAG IP Detect Kit

Immunoprecipitation Detection Kit (Product Code COIP-D)

The Immunoprecipitation Detection Kit provides all of the unique reagents required to detect protein-protein interactions using a plate-based colorimetric assay. The substrate p-Nitrophenyl Phosphate Disodium (pNPP) is hydrolyzed by Anti-c-Myc Alkaline Phosphatase-conjugated antibody to produce a yellow-colored solution that can be read spectrophotometrically at 405 nm. Tris Buffered Saline (pH 8.0) with 3% Nonfat Milk is used to dilute the Anti-c-Myc Alkaline Phosphatase Conjugate. The pNPP reaction is terminated with 3 N NaOH and then read at 405 nm. The SIGMA FAST™ pNPP tablet set provided in the kit produces a ready-to-use solution after the addition of 5 ml of distilled or deionized water.

Reagents Provided (sufficient for 96 samples)
Kit Components Product Code
Monoclonal Anti-c-Myc, Clone 9E10, Alkaline Phosphatase Conjugate A 5963 1 x 0.25 ml
Tris Buffered Saline, pH 8.0, with 3% Nonfat Milk T 8793 1 packet
SIGMA FAST™ p-Nitrophenyl Phosphate Tablets N 1891 5 tablet sets