Stem Cell Culture Basics: Media, Cell Lines and Culture Reagents

Stem cell culture

Stem cells have the unique ability to self-renew or to differentiate into various cell types in response to appropriate signals. These properties provide stem cells with unique capabilities for tissue repair, replacement, and regeneration. Accordingly, human stem cells are of special interest in medical research. Embryonic stem cells have the ability to differentiate into more cell types than adult stem cells. Differentiation is triggered by various factors in vivo, some of which can be replicated in in vitro stem cell cultures. The nature of stem cells necessitates the use of special stem cell culture media and reagents. Since suboptimal media may change the differentiation potential of stem cells, it is vital to choose the correct stem cell validated media and reagents at the start of your research process. Use the selection guide provided below during the planning phase of your research project. With the correct reagents, stem cells can be cultured like any other cell lines. They are no more or less finicky. Some stem cell lines are immortal and can be cultured indefinitely in the lab, but many are not. Be sure to choose the right cell line for your purpose!

Recently, there have been advances in the realm of the stem cell research due to the advent of CRISPR genome editing technology and more advanced 3D cell culture techniques. These cell culture protocols, such as organoid cell cultures, have provided more predictive in vitro cellular “Disease-in-a-Dish” models. Combining our CRISPR, ZFN gene editing, and stem cell expertise, MilliporeSigma now offers novel stem cell lines, optimized media, and innovative kits for all areas of stem cell biology, including induced pluripotent stem cells (iPSCs), neural, mesenchymal and hematopoietic stem cell culture. In addition to our expansive portfolio of assay ready stem cells, serum-free cell culture media, and 3D culture solutions, we offer custom engineered stem cell lines through our easy-to-use Cell Design Studio. Choose your favorite host or iPSC line and watch our dedicated team of scientists knock-out, modify, or knock-in your gene of interest.

 

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