HyStem™ Cell Culture

What is Endotoxin?

Brief Summary
Endotoxins are small, stable, bacterially-derived hydrophobic molecules which can easily contaminate labware and whose presence can significantly impact both in vitro and in vivo experiments.2 Their presence is detected by the limulus amebocyte lysate (LAL) assay which can detect down to 0.01 Endotoxin Units (EU)/ml. Thorough cleanliness in labware, raw materials, and in lab technique is required to substantially reduce endotoxin levels. Sigma’s ultrapure HyStem line of products is produced according to these guidelines, yielding a product that is suitable for demanding in vitro and in vivo experiments.