Serum-Free Stem Cell Media & Supplements

Historically, fetal bovine serum (FBS) has been the supplement of choice for in vitro mammalian cell culture including stem cells. However, due to the undefined composition of FBS, researchers have transitioned to more chemically defined serum-free and xeno-free media and supplements for stem cell research applications. To realize the true potential of stem cell therapy highly performing serum-free media (SFM) is critical for success.

Advantages of Serum-Free Media:

  • Consistency: Defined media composition ensures lot-to-lot consistency.
  • Performance: High biological activity of components produces superior performance.
  • Traceability: Animal-origin-free components ensures global regulatory compliance.
  • Availability: Stable supply and price.

Sigma-Aldrich offers high performance ready-to-use proprietary serum-free stem cell media for a variety of stem cell types including Human ES/iPS, Neural (NSC), Mesenchymal (MSC) and Hematopoietic (HSC) stem cells. These media are highly quality controlled to ensure performance and consistency. All Stemline media are produced in a GMP state-of-the-art facility with available Device Master Files (DMF).

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Serum-Free Stem Cell Expansion Media

Cell Type Product Description Ordering Info
Human ES/IPS Cell PluriSTEM Human ES/iPS Medium SCM130
  PluriSTEM-XF Human ES/iPS Medium SCM132
Mouse Embryonic Stem Cell ESGRO Complete PLUS Clonal Grade Medium SF-001-500P
  ESGRO-2i Medium SF-016-200
Neural Stem Cell Stemline Neural Stem Cell Expansion Medium S3194-500ML
  ReNcell NSC Maintenance Media SCM005
Mesenchymal Stem Cell Stemline Mesenchymal Stem Cell Expansion Medium S1569-1L
  PLTMax Human Platelet Lysate SCM141, SCM142
  PLTGold Human Platelet Lysate (Heparin-Free) SCM151, SCM152
Hematopoietic Stem Cell Stemline II Hematopoietic Stem Cell Expansion Medium S0192-500ML
Cancer Stem Cell 3dGRO Spheroid Media S3077

Sigma-Aldrich is a leader in cell culture qualified chemicals and media supplements. When developing serum-free media for stem cell applications high quality supplements such as serum albumin proteins, hormones, fatty-acids, vitamins, trace elements, growth factors and ECM proteins are often used to replace critical FBS components.

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Common Serum-Free Media Supplements

Supplement Type Product Description Ordering Info
Complete Supplements NDiff Neuro-2 Medium Supplement (200x) SCM012
  NDiff Neuro-27 Medium Supplement (100x) SCM013
  GS21™ Supplement (50X) G0800
  ITS Liquid Media Supplement (100×) I3146-5ML
Serum Proteins Human Serum Albumin A9731
  Bovine Serum Albumin A9418
  Bovine Serum Albumin Fraction V, fatty acid free 10775835001
  Apo-Transferrin Human T1147
  Holo-Transferrin human T0665
Hormones Insulin, Recombinant Human I9278
  Insulin Solution from Bovine Pancreas I0516
  Hydrocortisone Solution, Animal Component-free H 6909
  Progesterone P6149
Fatty Acids and Lipids Fatty Acid Supplement F7050
  Chemically Defined Lipid Mixture L0288
  Cholesterol C1231
  Linoleic Acid L8384
  Oleic Acid O3008
  Ethanolamine E0135
  Putrescine P6024
Vitamins and Trace Elements Sodium Selenite S9133
  D-(+)-Glucose G7021
  Alanyl-Glutamine A8185
  MEM Non-essential Amino Acid Solution (100×) M7145
  L-Ascorbic acid A4403
  L-Glutathione reduced G6013
  Retinol (Vitamin A) R7632
  3,3′,5-Triiodo-L-thyronine Sodium Salt T5516
  Sodium Pyruvate P5280
Growth Factors and Cytokines EGF, Human Recombinant E9644
  FGF, Human Recombinant F9786
  VEGF, Human Recombinant V7259
  IGF, Human Recombinant I3769
  PDGF-BB, Human Recombinant Animal Free SRP3138
  TGF-β1, Human Recombinant Animal Free GF346
ECM Proteins Fibronectin from human plasma F2006
  Laminin from Engelbreth-Holm-Swarm Sarcoma L2020
  Laminin from human placenta L6274
  Vitronectin from human plasma V8379
  MaxGel™ ECM E0282