The full medium composition is in the datasheet that can be downloaded from the webpage:
1.07700
RAPPAPORT-VASSILIADIS-Soya Broth
GranuCult® prime, according to ISO 6579, ISO 19250, EN 15215, USDA-FSIS, granular, selective for Salmonella spp.
Synonym(s):
Salmonella Enrichment Broth acc. to Rappaport and Vassiliadis, RVS broth (base), Rappaport Vassiliadis Salmonella Enrichment Broth
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| Size/SKU | Availability | Price |
|---|---|---|
500 g | Please contact Customer Service for Availability | 2 670,00 CZK |
About This Item
NACRES:
NA.74
UNSPSC Code:
41106213
Manufacturer/tradename:
GranuCult® prime
Agency:
EN 15215, ISO 19250, USDA-FSIS, according to ISO 6579
Ph:
5.0-5.4 (25 °C, 26.6 g/L in H2O, after autoclaving)
Form:
granular
Suitability:
selective for Salmonella spp.
Application(s):
food and beverages
microbiology
microbiology
2 670,00 CZK
Please contact Customer Service for Availability
Consider alternative product 17173-500G
Quality Segment
agency
EN 15215, ISO 19250, USDA-FSIS, according to ISO 6579
form
granular
packaging
pack of 500 g
manufacturer/tradename
GranuCult® prime
technique(s)
microbiological culture: suitable
pH
5.0-5.4 (25 °C, 26.6 g/L in H2O, after autoclaving)
bulk density
700 kg/m3
application(s)
food and beverages
microbiology
storage temp.
15-25°C
suitability
selective for Salmonella spp.
General description
RVS (RAPPAPORT-VASSILIADIS-Soya) broth is a selective medium for the enrichment and isolation of Salmonella. It is a modification of the original Rappaport medium, where soya peptone is used instead of tryptone (peptone from casein) to improve recovery rates of Salmonella. The efficiency of RVS broth for selection of Salmonella is based on (a) the ability of Salmonella sp. to multiply at relatively high osmotic pressures, at relatively low pH values, at a high temperature, and with modest nutritional requirements and (b) the suppression of the toxic effect of malachite green towards Salmonellae in the presence of magnesium chloride.
Application
RVS (RAPPAPORT-VASSILIADIS-Soya) broth (base) is used for the selective enrichment of Salmonella from food and animal feed, water, and other materials.
Analysis Note
Appearance (clearness): clear
Appearance (colour): darkblue
pH-value (25 °C): 5.0 - 5.4
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Mixed inoculum of Salmonella, E.coli and P.aeruginosa
Additionaly monoculture of E.coli and E.faecalis
Confirmation on XLD agar resp. Tryptic soy agar
Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): ≤ 100
Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≥ 1000
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≥ 1000
Inoculum on reference medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): ≥ 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)): ≥ 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): ≥ 1000
Growth on XLD agar (Salmonella typhimurium ATCC 14028 (WDCM 00031)): >10 colonies
Growth on XLD agar (Salmonella enteritidis ATCC 13076 (WDCM 00030)): >10 colonies
Colonies (Salmonella typhimurium ATCC 14028 (WDCM 00031)): colourless with black centre
Colonies (Salmonella enteritidis ATCC 13076 (WDCM 00030)): colourless with black centre
Growth on Tryptic soy agar (Escherichia coli ATCC 25922 (WDCM 00013)): <100 colonies
Growth on Tryptic soy agar (Escherichia coli ATCC 8739 (WDCM 00012)): <100 colonies
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 29212 (WDCM 00087)): <10 colonies
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 19433 (WDCM 00009)): <10 colonies
Incubation:
RVS broth: 24 ± 3 hours at 41.5 ± 1 °C aerobic
Confirmation on XLD agar resp. Tryptic Soy Agar: 24 ± 3 hours at 37 ± 1 °C aerobic
Appearance (colour): darkblue
pH-value (25 °C): 5.0 - 5.4
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Mixed inoculum of Salmonella, E.coli and P.aeruginosa
Additionaly monoculture of E.coli and E.faecalis
Confirmation on XLD agar resp. Tryptic soy agar
Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031)): ≤ 100
Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030)): ≤ 100
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013)): ≥ 1000
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012)): ≥ 1000
Inoculum on reference medium (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): ≥ 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 29212 (WDCM 00087)): ≥ 1000
Inoculum on reference medium (Enterococcus faecalis ATCC 19433 (WDCM 00009)): ≥ 1000
Growth on XLD agar (Salmonella typhimurium ATCC 14028 (WDCM 00031)): >10 colonies
Growth on XLD agar (Salmonella enteritidis ATCC 13076 (WDCM 00030)): >10 colonies
Colonies (Salmonella typhimurium ATCC 14028 (WDCM 00031)): colourless with black centre
Colonies (Salmonella enteritidis ATCC 13076 (WDCM 00030)): colourless with black centre
Growth on Tryptic soy agar (Escherichia coli ATCC 25922 (WDCM 00013)): <100 colonies
Growth on Tryptic soy agar (Escherichia coli ATCC 8739 (WDCM 00012)): <100 colonies
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 29212 (WDCM 00087)): <10 colonies
Growth on Tryptic soy agar (Enterococcus faecalis ATCC 19433 (WDCM 00009)): <10 colonies
Incubation:
RVS broth: 24 ± 3 hours at 41.5 ± 1 °C aerobic
Confirmation on XLD agar resp. Tryptic Soy Agar: 24 ± 3 hours at 37 ± 1 °C aerobic
Other Notes
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
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This Item | |||
|---|---|---|---|
| manufacturer/tradename GranuCult® | manufacturer/tradename ReadyTube® | manufacturer/tradename GranuCult® prime | manufacturer/tradename GranuCult® prime |
| technique(s) microbiological culture: suitable | technique(s) - | technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable |
| form medium granules (dehydrated (DCM)) | form liquid (ready-to-use) | form granular | form granular |
| application(s) food and beverages | application(s) bioburden testing | application(s) food and beverages | application(s) food and beverages |
| suitability Salmonella spp. | suitability selective for Salmonella spp. | suitability Listeria Spp. | suitability Listeria Spp. |
| agency ISO 6579 | agency ISO 19250, ISO 6579, ISO 6785 I IDF 93, ISO FDIS 6579-1 | agency APHA, according to USDA-FSIS | agency APHA, according to USDA-FSIS |
Storage Class
13 - Non Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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