This kit has not been tested on cell or tissue lysates, and its effectiveness for this sample type is unknown. It is essential to conduct tests on this specific sample. Lysates can be prepared by dissolving directly in the assay buffer, which contains detergent. The required amounts of tissue, cells, and assay buffer should be determined by the end user. As a starting guideline, use 1 million cells (or a small amount of tissue) in 100 µL of assay buffer. Homogenize with a Dounce homogenizer, centrifuge at 10,000 g for 5 minutes, and use the supernatant for the assay.
MAK366
Total Sulfite Assay Kit (Colorimetric)
sufficient for 100 colorimetric tests
Synonym(s):
Sulfite Colorimetric Assay Kit
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About This Item
NACRES:
NA.84
UNSPSC Code:
12161503
Pricing and availability is not currently available.
usage
sufficient for 100 colorimetric tests
application(s)
cosmetics
food and beverages
detection method
colorimetric
relevant disease(s)
pulmonary disorders
storage temp.
−20°C
General description
Sulfites/sulphites are substances that occur naturally in the human body due to the metabolism of amino acids containing sulfur in their side chains. They are readily oxidized to sulfates via enzymatic reactions and excreted in urine at the rate of 1000 mg/day. Sulfites are often considered as allergens due to adverse symptoms observed in asthmatic patients. Exogenous sources of sulphites include polluted air, food and beverages containing sulphur dioxide (SO2). SO2 is a molecule that easily reacts with several small compounds including aldehydes, ketones, anthocyanins, cobalamine, thiamine, NAD, flavins, among others. SO2 also interacts with cysteine residues in proteins and promotes cross-linking. Sulfites are also used as regulated food preservatives/additives/enhancers in dried fruits, wine, beer, etc. They are considered as GRAS (Generally Recognized as Safe) in food and beverages. In wine, SO2 reacts with sugars, aldehydes and anthocyanins. The term sulfite is used for all sulfite-derived molecules: bisulfites, metasulfites, and SO2. Total sulfite is defined as the sum of bound and free sulfite.
Application
Suitable for the measurement of sulfite in various food and beverage samples (for example wine, dairy, canned products, juices, dried fruit, etc.).
Biochem/physiol Actions
The Total Sulfite Assay Kit is a simple and sensitive assay to detect small concentrations of sulfites in a variety of food and beverage samples. This assay is based on the oxidation of sulfite to sulfate producing a stable signal at A570 nm, which is directly proportional to the amount of sulfite in the sample. This assay is very sensitive and can detect as low as 20 μM of sulfite in a variety of samples
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 3
Storage Class
10 - Combustible liquids
flash_point_f
188.6 °F
flash_point_c
87 °C
wgk
WGK 3
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what is the best way to lyse cells/tissue for using this kit?
1 answer-
Helpful?
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What is the best way to lyse cells/tissue for using this kit?
1 answer-
This kit has not been tested on cell or tissue lysates, and its effectiveness for this sample type is unknown. It is essential to conduct tests on this specific sample. Lysates can be prepared by dissolving directly in the assay buffer, which contains detergent. The required amounts of tissue, cells, and assay buffer should be determined by the end user. As a starting guideline, use 1 million cells (or a small amount of tissue) in 100 µL of assay buffer. Homogenize with a Dounce homogenizer, centrifuge at 10,000 g for 5 minutes, and use the supernatant for the assay.
Helpful?
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How should sucrose samples be prepared to test sulfite content with kit MAK366?
1 answer-
For samples like sucrose pellets, it is advisable to dissolve or dilute them in the assay buffer and then utilize them in the assay. The sucrose should be soluble in the buffer. Metabolites present in food samples might notably impede the signal. Hence, it is suggested to dilute the samples with Sulphite Assay Buffer. In case interference persists in the diluted samples, it is recommended to prepare parallel sample well(s) as sample background control(s) and adjust the volume to 50 µl/well with Sulphite Assay Buffer. It is advised to conducting a pilot experiment and testing several samples to ensure that the readings fall within the standard curve range.
Helpful?
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