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PROTSIL2

ProteoSilver Plus Silver Stain Kit

Synonym(s):

protein stain, silver stain

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1 KIT

$418.00

$418.00


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About This Item

NACRES:
NA.32
UNSPSC Code:
41105322

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usage

 kit sufficient for 25 mini-gels (10 × 10 cm)

Quality Level

technique(s)

protein staining: suitable

storage temp.

20-25°C

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This Item
PROTSIL1S5692HT100A
technique(s)

protein staining: suitable

technique(s)

protein staining: suitable

technique(s)

protein staining: suitable

technique(s)

-

usage

 kit sufficient for 25 mini-gels (10 × 10 cm)

usage

 kit sufficient for 25 mini-gels (10 × 10 cm)

usage

-

usage

-

storage temp.

20-25°C

storage temp.

-

storage temp.

-

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

500

General description

Conveniently packaged, extremely stable, and highly sensitive, ProteoSilver Plus Silver Stain Kit is an ideal product for any protein scientist. Similar to ProteoSilver (PROTSIL1), this kit contains prepared solutions of silver staining reagents along with detailed instructions to achieve optimal results. With a detection limit of 0.1 ng/mm2 of protein (BSA) and an extremely low background, ProteoSilver Plus leads to superior detection of low abundance proteins.
ProteoSilver Plus is also MALDI-MS compatible and does not contain glutaraldehyde, which crosslinks lysine residues, resulting in inaccurate spectra. ProteoSilver Plus Silver Stain Kit contains two additional reagents for destaining an excised protein spot, for those wishing to perform further characterization through tryptic digestion and MALDI-MS analysis.

Application

ProteoSilver Silver Stain Kit has been used for staining of the gels for visualizing proteins. It has also been used for destaining of the protein of interest using destainer provided in the kit for mass spectroscopic analysis.

Features and Benefits

  • Premixed and preweighed solutions reduce time and cost of purchasing and preparing individual components
  • Optimized protocols help establish conditions for best results
  • High sensitivity and low background ensure very low abundance proteins can be detected and resolved from other proteins
  • MALDI Compatible - Protein spots of interest can be further characterized by mass spectrometry
  • Room Temperature Stability allows easy and convenient storage

Legal Information

ProteoSilver is a trademark of Sigma-Aldrich Co. LLC

signalword

Danger

Hazard Classifications

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Repr. 1B - Skin Corr. 1A - Skin Sens. 1 - STOT SE 2 - STOT SE 3

target_organs

Eyes,Central nervous system, Respiratory system

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

149.0 °F - closed cup

flash_point_c

65 °C - closed cup


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K P Aggarwal et al.
International braz j urol : official journal of the Brazilian Society of Urology, 39(1), 128-136 (2013-03-16)
Proteins constitute a major portion of the organic matrix of human calcium oxalate (CaOx) renal stones and the matrix is considered to be important in stone formation and growth. The present study evaluates the effect of these proteins on oxalate
Kanu Priya Aggarwal et al.
PloS one, 8(7), e69916-e69916 (2013-07-31)
The increasing number of patients suffering from urolithiasis represents one of the major challenges which nephrologists face worldwide today. For enhancing therapeutic outcomes of this disease, the pathogenic basis for the formation of renal stones is the need of hour.
Effect of biomolecules from human renal matrix of calcium oxalate monohydrate (CaOx) stones on in vitro calcium phosphate crystallization.
Pathak P, et al.
International Brazilian Journal of Urology : Official Journal of the Brazilian Society of Urology, 36, 621-628 (2010)
2D map of proteins from human renal stone matrix and evaluation of their effect on oxalate induced renal tubular epithelial cell injury.
Aggarwal KP, et al.
International Brazilian Journal of Urology : Official Journal of the Brazilian Society of Urology, 39, 128-136 (2013)
Priyadarshini Pathak et al.
International braz j urol : official journal of the Brazilian Society of Urology, 36(5), 621-628 (2010-11-04)
Investigate the activity of high and low molecular weight biomolecules present in the matrix of human calcium oxalate (CaOx) stones not only on the initial mineral phase formation of calcium and phosphate (CaP) but also on its growth and demineralization

Articles

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

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Global Trade Item Number

SKUGTIN
PROTSIL2-1KT04061836687014

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