04-790
Anti-dimethyl-Histone H3 (Lys4) Antibody, clone AW30, rabbit monoclonal
culture supernatant, clone AW30, Upstate®
동의어(들):
H3K4me2, Histone H3 (di methyl K4)
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크기 선택
보기 변경
| 팩 크기/SKU | 재고 정보 | 가격 (VAT 별도) |
|---|---|---|
100 μL | 예상 입고일2026년 9월 11일재고 수량 조회Songdo warehouse | ₩806,012 |
제품정보 (DICE 배송 시 비용 별도)
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
AW30, monoclonal
Species reactivity:
chicken, human
Application:
ChIP, DB, WB, inhibition assay
Citations:
12
biological source
rabbit
Quality Segment
antibody form
culture supernatant
antibody product type
primary antibodies
clone
AW30, monoclonal
species reactivity
chicken, human
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable (ChIP-seq), dot blot: suitable, inhibition assay: suitable (peptide), western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
dimethylation (Lys4)
Gene Information
human ... H3C1(8350)
General description
Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle. Di- and trimethylation of histone H3 at Lys4 correlates with transcriptional activity of many genes. Dimethylation of Histone H3 at Lys4 occurs at both active and inactive euchromatic regions but not in silent heterochromatic sites, whereas trimethylation at Lys4 is present exclusively at active genes.
~17kDa
Immunogen
KLH-conjugated, synthetic peptide containing the sequence …Tme2KQT… in which me2K corresponds to dimethyl-lysine at residue 4 of human histone H3.
Application
Western Blot Analysis:
A 1:1000-1:2000 dilution of this lot detected dimethyl histone H3 (Lys4) in HeLa acid extracts.
Western Blot Analysis:
A 1:2,000 to 1:10,000 dilution of a previous lot detected methylated histone H3 in acid extracted proteins from HeLa cells. The antibody did not detect unmethylated recombinant Histone H3.
ChIP-seq Analysis:
Representative lot data. Chromatin immunoprecipitation was performed using the Magna ChIP
Dot Blot Analysis:
Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665), which contain histone peptides with various modifications were probed with Cat. No 04-790, Anti-dimethyl Histone H3 (Lys4), clone AW30 at 1:1000 dilution. Proteins were visualized using a Donkey anti-Rabbit IgG conjugated to HRP and a chemiluminescence detection system.
Peptide Inhibition Analysis:
2 μM of a histone H3 peptide containing dimethyl-lysine 4 abolished detection of histone H3 by a previous lot of antibody in immunoblot analysis of HeLa acid extracts. No signal reduction was observed with histone H3 peptides containing either monomethyl or trimethyl-lysine 4 modifications
A 1:1000-1:2000 dilution of this lot detected dimethyl histone H3 (Lys4) in HeLa acid extracts.
Western Blot Analysis:
A 1:2,000 to 1:10,000 dilution of a previous lot detected methylated histone H3 in acid extracted proteins from HeLa cells. The antibody did not detect unmethylated recombinant Histone H3.
ChIP-seq Analysis:
Representative lot data. Chromatin immunoprecipitation was performed using the Magna ChIP
Dot Blot Analysis:
Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665), which contain histone peptides with various modifications were probed with Cat. No 04-790, Anti-dimethyl Histone H3 (Lys4), clone AW30 at 1:1000 dilution. Proteins were visualized using a Donkey anti-Rabbit IgG conjugated to HRP and a chemiluminescence detection system.
Peptide Inhibition Analysis:
2 μM of a histone H3 peptide containing dimethyl-lysine 4 abolished detection of histone H3 by a previous lot of antibody in immunoblot analysis of HeLa acid extracts. No signal reduction was observed with histone H3 peptides containing either monomethyl or trimethyl-lysine 4 modifications
Anti-dimethyl-Histone H3 (Lys4) Antibody, clone AW30 is a rabbit monoclonal antibody for detection of dimethyl-Histone H3 (Lys4) also known as H3K4me2, Histone H3 (di methyl K4) & has been validated in WB, ChIP, ChIP-seq, DB, PIA, Mplex.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Biochem/physiol Actions
Histone H3 dimethylated on lysine 4
The immunizing sequence is conserved from Tetrahymena to human, so broad species cross-reactivity is expected.
Physical form
Cultured supernantant in 0.05% sodium azide
Preparation Note
Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Analysis Note
Control
HeLa acid extracts
HeLa acid extracts
routinely evaluated by immunoblot on acid-extracted proteins from HeLa cells; the antibody did not detect unmethylated recombinant Histone H3 (Catalog #14-494)
Other Notes
Replaces: 05-790
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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이 품목 | |||
|---|---|---|---|
| description culture supernatant, clone AW30, Upstate® | description culture supernatant, clone AW304, Upstate® | description culture supernatant, clone 614M, from rabbit | description clone CMA304, from mouse |
| species reactivity chicken, human | species reactivity vertebrates, human | species reactivity vertebrates | species reactivity human, vertebrates |
| antibody form culture supernatant | antibody form culture supernatant | antibody form culture supernatant | antibody form purified antibody |
| clone AW30, monoclonal | clone AW304, monoclonal | clone 614M, monoclonal | clone CMA304, monoclonal |
| biological source rabbit | biological source rabbit | biological source rabbit | biological source mouse |
| Gene Information human ... H3C1(8350) | Gene Information human ... H3C1(8350) | Gene Information human ... H3C1(8350) | Gene Information human ... H3C1(8350) |
| isotype IgG | isotype IgG | isotype IgG | isotype IgG1κ |
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