This product is offered as ascites fluid and is not a purified antibody. The antibody concentration is not determined. Dilutions factors are established for Western blot and Flow Cytometry. This information is available in the product Certificate of Analysis. Please see the link below to access the lot-specific Certificate:
https://www.sigmaaldrich.com/product/mm/mabc710#product-documentation
MABC710
Anti-TGFBR3, clone 1C5H11 Antibody
ascites fluid, clone 1C5H11, from mouse
Synonym(s):
Transforming growth factor beta receptor type 3, TGF-beta receptor type 3, TGFR-3, Betaglycan, Transforming growth factor beta receptor III, TGF-beta receptor type III, TGFBR3
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| Size/SKU | Availability | Price |
|---|---|---|
100 μL | Please contact Customer Service for Availability | $706.00 |
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
1C5H11, monoclonal
Application:
ELISA, FACS, WB
Citations:
-
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
1C5H11, monoclonal
species reactivity
human, mouse
technique(s)
ELISA: suitable, flow cytometry: suitable, western blot: suitable
isotype
IgG2b
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... TGFBR3(7049)
General description
Transforming growth factor beta receptor type 3 (TGF-beta receptor type 3 or TGFR-3), also known as Betaglycan or Transforming growth factor beta receptor III (TGF-beta receptor type III), and encoded by the gene name TGFBR3, is a membrane proteoglycan that often functions as a co-receptor with other TGF-beta receptor superfamily members. Ectodomain shedding produces soluble TGFBR3, which may inhibit TGFB signaling. Decreased expression of this receptor has been observed in various cancers. TGFBR3 is extensively modified by glycosaminoglycan groups (GAG).
~93 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Immunogen
Purified recombinant fragment of human TGFBR3 expressed in E. Coli
Application
Anti-TGFBR3, clone 1C5H11 Antibody is an antibody against TGFBR3 for use in western blotting, flow cytometry & ELISA.
Flow Cytometry Analysis: A 1:200-1:400 dilution from a representative lot detected TGFBR3 in MOLT4 cells.
ELISA: A representative lot was used on Control Antigen (100 ng) and Antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
ELISA: A representative lot was used on Control Antigen (100 ng) and Antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Tumor Markers
Tumor Markers
Physical form
Mouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.
Unpurified
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Western Blotting in Jurkat, HeLa, MCF-7, F9, SK-N-SH and NIH/3T3 cell lysates.
Western Blotting Analysis: A 1:500-1:2,000 dilution of this antibody detected TGFBR3 in Jurkat, HeLa, MCF-7, F9, SK-N-SH and NIH/3T3 cell lysates.
Western Blotting Analysis: A 1:500-1:2,000 dilution of this antibody detected TGFBR3 in Jurkat, HeLa, MCF-7, F9, SK-N-SH and NIH/3T3 cell lysates.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| antibody form ascites fluid | antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form affinity isolated antibody |
| species reactivity human, mouse | species reactivity human, mouse, rat | species reactivity mouse, rat, human | species reactivity mouse, rat, human |
| Gene Information human ... TGFBR3(7049) | Gene Information human ... TGFBR3(7049) | Gene Information human ... TGFBR3(7049) | Gene Information human ... TGFBR2(7048) |
| biological source mouse | biological source rabbit | biological source rabbit | biological source rabbit |
| clone 1C5H11, monoclonal | clone polyclonal | clone polyclonal | clone polyclonal |
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Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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