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Roche

KAPA2G Fast PCR Kit

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250 U
Please contact Customer Service for Availability
$284.00

About This Item

NACRES:
NA.54
UNSPSC Code:
41106300

$284.00

Please contact Customer Service for Availability
​
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usage

sufficient for 200 reactions, sufficient for 500 reactions

shelf life

≤18 mo.

feature

Fast PCR, Multiplex PCR, dNTPs included: no, hotstart: no

packaging

pkg of 100 U (KK5020), pkg of 250 U (KK5021)

manufacturer/tradename

Roche

technique(s)

PCR: suitable

input

purified DNA

storage temp.

−20°C

General description

KAPA2G Fast DNA Polymerase is a second-generation (2G) enzyme, engineered for higher processivity and speed, offering significantly faster extension rates than wild-type Taq polymerase. In addition to speed, KAPA2G Fast provides higher yields and sensitivity than competitor enzymes across a broad range of targets. KAPA2G Fast DNA Polymerase Kits are designed for fast PCR, in which total reaction times are 20–70% shorter than those of conventional PCR assays performed with wild-type Taq DNA polymerase. This can be achieved without sacrificing reaction performance, and does not require specialized PCR consumables or thermocyclers.

Application

KAPA2G Fast PCR Kit is suitable for:
  • routine polymerase chain reaction (PCR)
  • multiplex PCR
  • rapid photo PCR samples [1]
  • Fast PCR
  • genotyping

Biochem/physiol Actions

DNA fragments produced with KAPA2G Fast DNA Polymerase share the same characteristics as DNA fragments generated with wild-type Taq DNA polymerase and may be used for routine downstream analyses or applications, including restriction enzyme digestion, cloning and sequencing. Like wild-type Taq, KAPA2G Fast has 5′→3′ polymerase and 5′→3′ exonuclease activities, but no 3′→5′ exonuclease (proofreading) activity. The fidelity of KAPA2G Fast is similar to that of wild-type Taq; it has an error rate of approximately 1 error per 1.7 x 105 nucleotides incorporated.

Features and Benefits

Reduce cycling times up to 75% :
  • Extension times as low as 1 sec per kilobase
  • Broad coverage of both AT- and GC-rich targets

High speed, higher performance :
  • Single copy sensitivity from complex targets
  • Increased yields

Quick Notes :
  • KAPA2G Fast PCR Kits contain the engineered KAPA2G Fast DNA Polymerase, developed for fast PCR.
  • Use 1 sec extension time for amplicons <1 kb and 15 sec/kb for longer amplicons, and save 20–70% of total reaction time.
  • No need for specialized instrumentation or PCR consumables.
  • Optimized buffer system offers improved yields, specificity and sensitivity, facilitating efficient primer annealing across a wide range of primer lengths, GC contents, and melting temperatures.
  • Use 0.5 U KAPA2G Fast DNA Polymerase per 25 μL reaction, or less for smaller volumes.
  • For high reaction efficiency, do not exceed 25 μL reaction volumes.
  • KAPA2G Buffer A contains 1.5 mM MgCl2 at 1X.
  • Reaction products are 3′-dA-tailed and may be cloned into TA cloning vectors.

Preparation Note

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage. Provided that all components have been handled carefully and not contaminated, the kit is not expected to be compromised if left (unintentionally) at room temperature for a short period of time (up to 3 days). Long-term storage at room temperature and 4°C is not recommended. Please note that reagents stored at temperatures above -20°C are more prone to degradation when contaminated during use, and therefore storage at such temperatures is at the user′s own risk.

Analysis Note

Each batch of KAPA2G Fast DNA Polymerase is confirmed to contain <2% contaminating protein (Agilent Protein 230 Assay). KAPA2G Fast ReadyMix is subjected to stringent quality control tests, is free of contaminating exo- and endonuclease activity, and meets strict requirements with respect to DNA contamination levels.

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

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technique(s)

PCR: suitable

technique(s)

PCR: suitable

technique(s)

PCR: suitable

technique(s)

PCR: suitable

usage

sufficient for 200 reactions, sufficient for 500 reactions

usage

sufficient for 500 reactions

usage

sufficient for 1000 reactions, sufficient for 200 reactions, sufficient for 500 reactions

usage

sufficient for 1000 reactions, sufficient for 500 reactions

input

purified DNA

input

purified DNA

input

purified DNA

input

purified DNA

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

feature

Fast PCR, dNTPs included: no, Multiplex PCR, hotstart: no

feature

Fast PCR, Multiplex PCR, dNTPs included, hotstart: no

feature

Fast PCR, dNTPs included, hotstart

feature

Fast PCR, dNTPs included, hotstart

packaging

pkg of 250 U (KK5021), pkg of 100 U (KK5020)

packaging

pkg of 100 U (KK5008), pkg of 250 U (KK5009)

packaging

pkg of 100 U (KK5530), pkg of 250 U (KK5502), pkg of 500 U (KK5500)

packaging

pkg of 250 U (KK5512), pkg of 500 U (KK5510)

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Kit Components Only

Product No.
Description
  • KAPA2G Fast DNA Polymerase 5 U/µL
  • 5X KAPA2G Buffer A
  • KAPA MgCl2 25 mM

pictograms

Exclamation markHealth hazard
GHS07,GHS08

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 3 - STOT SE 2

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
112903
111463
105070
105069
006346-7-1

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Articles

KAPA2G Fast PCR Kits FAQ

Fast PCR reduces reaction time, enhancing efficiency with optimized cycling parameters.

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Global Trade Item Number

SKUGTIN
KK502004061834429562
KK502104061834429579