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KAPA PROBE FAST

Name: KAPA PROBE FAST
Brand: ROCHE
Price: 478 PLN

suitable for qPCR, 2 ×, Universal

Synonym(s):

qPCR

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Pack Size	Availability	Price
1 mL	Please contact Customer Service for Availability	PLN 478.00
5 mL	Please contact Customer Service for Availability	PLN 2,250.00
10 mL	Please contact Customer Service for Availability	PLN 4,300.00
50 mL	Please contact Customer Service for Availability	PLN 16,040.00

About This Item

NACRES:

NA.55

UNSPSC Code:

41106300

PLN 478.00

Please contact Customer Service for Availability

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usage

sufficient for 100 reactions, sufficient for 1000 reactions, sufficient for 500 reactions, sufficient for 5000 reactions

shelf life

≤12 mo.

feature

dNTPs included, hotstart

packaging

kit of 1 mL (100 x 20 μL rxn; KK4701), kit of 10 mL (1000 x 20 μL rxn; KK4703), kit of 5 mL (500 x 20 μL rxn; KK4702), kit of 50 mL (5000 x 20 μL rxn; KK4715)

manufacturer/tradename

Roche

concentration

2 ×

technique(s)

qPCR: suitable

input

purified DNA

compatibility

for use with Agilent Mx3000P, for use with Agilent Mx3005P, for use with Agilent Mx4000, for use with Applied Biosystems^® 5700, for use with Applied Biosystems^® 7000, for use with Applied Biosystems^® 7300, for use with Applied Biosystems^® 7500, for use with Applied Biosystems^® 7700, for use with Applied Biosystems^® 7900HT, for use with Applied Biosystems^® ViiA 7, for use with QuantStudio^™, for use with StepOne^™, for use with StepOnePlus^™

detection method

probe-based

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This Item	PFLRKB	PFABIKB	KK4752
Roche PFUKB KAPA PROBE FAST	Roche PFLRKB KAPA PROBE FAST	Roche PFABIKB KAPA PROBE FAST	Roche KK4752 KAPA PROBE FAST One-Step
technique(s) qPCR: suitable	technique(s) qPCR: suitable	technique(s) qPCR: suitable	technique(s) RT-qPCR: suitable
input purified DNA	input purified DNA	input -	input purified DNA
feature dNTPs included, hotstart	feature dNTPs included, hotstart	feature -	feature dNTPs included: no, hotstart: no
usage sufficient for 100 reactions, sufficient for 500 reactions, sufficient for 1000 reactions, sufficient for 5000 reactions	usage sufficient for 100 reactions, sufficient for 1000 reactions, sufficient for 500 reactions	usage -	usage sufficient for 500 reactions
shelf life ≤12 mo.	shelf life ≤12 mo.	shelf life ≤12 mo.	shelf life -
detection method probe-based	detection method probe-based	detection method -	detection method probe-based

General description

KAPA PROBE FAST qPCR Kits provide fast and reproducible results for all probe-based qPCR applications. Kits contain a ready-to-use master mix for highly sensitive and accurate real-time PCR using sequence-specific fluorogenic probe chemistries, including hydrolysis probes (e.g. TaqMan^®), FRET probes, and displacement probes (e.g. molecular beacons).

Application

KAPA PROBE FAST may be used for:

Real-time quantitative methylation-specific PCR (qMSP)[1]
Genotyping[2]
Gene expression analysis
Multiplex qPCR and qRT-PCR
SNP genotyping/allelic discrimination
Microarray validation

Features and Benefits

Accurately discriminate between alleles:

Exceptional differentiation of heterozygous and homozygous alleles
Discrete clusters in SNP genotyping assays

Achieve excellent reproducibility and efficiency:

Fast, high performance five-colored multiplex PCR
Similar abundance levels achieved with low copy number
Broad dynamic range of up to 10 orders of magnitude

Increase flexibility:

Compatible with both standard and fast cycling protocolsRetain accurate quantification

Quick Notes:

These kits are compatible with all fluorogenic probebased technologies, including hybridization probes (e.g., FRET), hydrolysis probes (e.g., TaqMan^®) and displacement probes (e.g., molecular beacons).
Initial denaturation for 20 sec at 95ºC is sufficient for enzyme reactivation; however, optimal denaturation of complex targets may require up to 3 min denaturation.
For 2-step cycling, use 30 sec combined annealing/ extension/data acquisition.
For 3-step cycling, use 20 sec for primer annealing and 1 sec for extension/data acquisition.
Do not exceed 25 μL volumes.

Preparation Note

ROX/fluorescein reference dyes (depending on kit configuration) are light sensitive. Exposure to direct light for an extended period of time will result in loss of fluorescent signal intensity. Avoid repeated freezing and thawing. Always ensure that the product has been fully thawed and mixed before use.

KAPA PROBE FAST qPCR Master Mix (2X) is stable through 30 freeze-thaw cycles. Ensure that all reagents are stored protected from light at -20°C when not in use. When protected from light, reagents are stable in the dark at 4°C for at least one week, and may be stored at this temperature for short-term use provided that they do not become contaminated with microbes and/or nucleases.

Analysis Note

KAPA PROBE FAST qPCR Master Mix (2X) is subjected to stringent quality control tests, is free of contaminating exoand endonuclease activity, and meets strict requirements with respect to DNA contamination levels.

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Legal Information

Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

QuantStudio is a trademark of IROA Technologies LLC

StepOne is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

StepOnePlus is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Kit Components Only

Product No.

Description

KAPA Taq HotStart^® DNA Polymerase

50X ROX^™ High Reference Dye

50X ROX^™ Low Reference Dye

reaction buffer

dNTPs

MgCl₂ 5 mM

wgk

WGK 1

Storage Class

12 - Non Combustible Liquids

flash_point_f

does not flash

flash_point_c

does not flash

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Voltage-gated sodium channel polymorphisms play a pivotal role in the development of oxaliplatin-induced peripheral neurotoxicity: results from a prospective multicenter study.

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A multiplex TaqMan qPCR assay for sensitive and rapid detection of phytoplasmas infecting Rubus species.

Holger Linck et al.

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Rubus stunt is an economically important disease in the production of raspberries, blackberries, and loganberries. A fast, sensitive, and reliable diagnosis of phytoplasmas, the causal agent of the disease, is of prime importance to stop its spread by vegetative propagation

Cell-free methylation markers with diagnostic and prognostic potential in hepatocellular carcinoma.

Lu C Y, et al.

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Molecular Analyses Define Vα7.2-Jα33+ MAIT Cell Depletion in HIV Infection: A Case-Control Study.

James E Ussher et al.

Medicine, 94(29), e1134-e1134 (2015-07-23)

Mucosal-associated invariant T (MAIT) cells are an abundant antibacterial innate-like lymphocyte population. There are conflicting reports as to their fate in HIV infection. The objective of this study was to determine whether MAIT cells are truly depleted in HIV infection.

Long non-coding RNA MEG3 functions as a competing endogenous RNA to regulate gastric cancer progression.

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Journal of Experimental & Clinical Cancer Research, 34(1), 79-79 (2015)

Articles

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An overview of directed evolution and the methods for generating proteins with optimized or entirely new functions.

Global Trade Item Number

SKU	GTIN
KK4703	04061838087072
KK4701	04061838087058
KK4702	04061838087065
KK4715	04061837897559

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