07373
Phalloidin–Atto 740
BioReagent, suitable for fluorescence
Synonym(s):
Atto 740–Phalloidin
Select a Size
| Size/SKU | Availability | Price |
|---|---|---|
10 nmol | Check Cart for Availability | ¥135,000 |
About This Item
product line
BioReagent
Quality Segment
assay
≥90.0% (HPLC)
form
powder
manufacturer/tradename
ATTO-TEC GmbH
transmittance
254 nm, 740 nm
fluorescence
λex 750 nm; λem 764 nm in 0.1 M phosphate pH 7.0
UV absorption
λ: 739 -745 nm Amax
suitability
suitable for fluorescence
storage temp.
−20°C
General description
Atto 740 is a pH sensitive product. While practically stable up to pH 7.4 (PBS-buffer), it slowly degrades at higher pH. If exposed to higher pH for coupling purposes, we recommend reducing the pH immediately after completion of the reaction.
Phalloidin is a fungal toxin isolated from the poisonous mushroom Amanita phalloides. Its toxicity is attributed to the ability to bind F actin in liver and muscle cells. As a result of binding phalloidin, actin filaments become strongly stabilized. Phalloidin has been found to bind only to polymeric and oligomeric forms of actin, and not to monomeric actin. The dissociation constant of the actin-phalloidin complex has been determined to be on the order of 3 x 10-8. Phalloidin differs from amanitin in rapidity of action; at high dose levels, death of mice or rats occurs within 1 or 2 hours. Fluorescent conjugates of phalloidin are used to label actin filaments for histological applications. Some structural features of phalloidin are required for the binding to actin. However, the side chain of amino acid 7 (g-d-dihydroxyleucine) is accessible for chemical modifications without appreciable loss of affinity for actin.
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Packaging
Legal Information
1 of 1
This Item | |||
|---|---|---|---|
| suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence |
| fluorescence λex 750 nm; λem 764 nm in 0.1 M phosphate pH 7.0 | fluorescence λex 700 nm; λem 719 nm in 0.1 M phosphate pH 7.0 | fluorescence - | fluorescence - |
| form powder | form powder | form solid | form - |
| Quality Level 100 | Quality Level - | Quality Level 100 | Quality Level 100 |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| assay ≥90.0% (HPLC) | assay ≥90% (HPLC) | assay ≥90.0% (HPLC) | assay ≥90.0% (HPLC) |
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
07373-10NMOL:
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