ATTO 647N is a stable chemical and photochemical dye. No negative impact is expected when storing this product at -20°C with glycerol and subjecting it to multiple freeze/thaw cycles.
76508
Atto 647N Protein Labeling Kit
BioReagent, suitable for fluorescence
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 kit | Check Cart for Availability | $821.00 |
About This Item
NACRES:
NA.32
UNSPSC Code:
12352108
Storage temp.:
2-8°C
product line
BioReagent
Quality Level
manufacturer/tradename
ATTO-TEC GmbH
fluorescence
λex 647 nm; λem 661 nm in 0.1 M phosphate buffer, pH 7.0 (recommended)
suitability
suitable for fluorescence
storage temp.
2-8°C
General description
This kit contains sufficient amounts of reactive dye, buffers and protein purification sets for performing five labeling reactions (1 mg protein each) and for the subsequent purification of the labeled protein.
Legal Information
This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.
1 of 1
This Item | |||
|---|---|---|---|
| manufacturer/tradename ATTO-TEC GmbH | manufacturer/tradename ATTO-TEC GmbH | manufacturer/tradename ATTO-TEC GmbH | manufacturer/tradename ATTO-TEC GmbH |
| fluorescence λex 647 nm; λem 661 nm in 0.1 M phosphate buffer, pH 7.0 (recommended) | fluorescence λex 633 nm; λem 661 nm in 0.1 M phosphate buffer, pH 7.0 (recommended) | fluorescence λex 544 nm; λem 576 nm in 0.1 M phosphate buffer, pH 7.0 (recommended) | fluorescence λex 488 nm; λem 520 nm in 0.1 M phosphate buffer, pH 7.0 (recommended) |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| product line BioReagent | product line BioReagent | product line BioReagent | product line BioReagent |
| suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence |
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Related Content
Protein labeling kits with Atto and Tracy dyes provide easy fluorescent labeling of purified proteins, enzymes, and antibodies.
Bryant L Doss et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(23), 12817-12825 (2020-05-24)
Morphogenesis, tumor formation, and wound healing are regulated by tissue rigidity. Focal adhesion behavior is locally regulated by stiffness; however, how cells globally adapt, detect, and respond to rigidity remains unknown. Here, we studied the interplay between the rheological properties
Anne Chalumeau et al.
Bio-protocol, 11(2), e3899-e3899 (2021-03-19)
β-hemoglobinopathies are severe genetic disorders characterized either by the abnormal synthesis of the adult β-globin chains of the hemoglobin (Hb) tetramer (βS-globin chains) in sickle cell disease (SCD) or by the reduced β-globin production in β-thalassemia. The identification and quantification
Alexandra Grubman et al.
Nature communications, 12(1), 3015-3015 (2021-05-23)
The role of microglia cells in Alzheimer's disease (AD) is well recognized, however their molecular and functional diversity remain unclear. Here, we isolated amyloid plaque-containing (using labelling with methoxy-XO4, XO4+) and non-containing (XO4-) microglia from an AD mouse model. Transcriptomics
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 76508-1KT | 04061823369831 |
-
Can we freeze/defreeAfter marking and storing the product at -20°C with glycerol, does the marking remain stable after multiple freeze/thaw cycles?
1 answer-
Helpful?
-
-
Is there an optimal volume of protein at c=2mg/ml (e.g. is it better to have 1 mg in 0.5ml PBS or 2mg in 1ml PBS). Incubation of 2h is performed on RT or +4C?
1 answer-
Per the product technical bulletin, the labeling reaction is sufficient for 1 mg of protein at a concentration of 2–10 mg/mL, and the two-hour incubation step is at room temperature.
Helpful?
-
-
Will this kit efficiently label protein on a much smaller scale of < 20ug of protein or at concentration below 2 mg/ml?
1 answer-
The dye should be reconstituted in 20 ul of DMSO and the total volume added to 1 mg of the antibody (at 2-10 mg/ml). If the amount of antibody is less, it needs to be at 2 mg/ml, and the amount of dye added to the antibody solution should be proportionally reduced. For example, if there is 200 ug of antibody (at 2 mg/ml), 4 ul of the DMSO solution of the dye should be added to the antibody. It's important for the user to use very accurate pipettes to avoid adding too much dye to the antibody, which could result in the labeling of the antibody in the antigen-binding site. If less protein is used, the amount of dye can be reduced. There is always an excess of dye to ensure labeling efficiency, and a part of the dye will be separated (as unbound dye) by a purification step afterward. The proteins need to have a minimum concentration due to the strong hydrolysis tendency of the NHS-functionality. If the protein solution is too dilute, the dye will be hydrolyzed before it reaches an amine-group of a protein.
Helpful?
-