Fortfahren mit
CAS9P
Cas9 plasmid
Größe auswählen
| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|---|---|---|
| 1 ea | Warenkorb auf Verfügbarkeit prüfen | CHF 254.00 |
Über diesen Artikel
recombinant
expressed in E. coli
Quality Level
packaging
vial of 50 μL
concentration
20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
promoter
Promoter name: CMV
selection
kanamycin
shipped in
dry ice
storage temp.
−20°C
Verwandte Kategorien
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| promoter Promoter name: CMV | promoter Promoter name: CMV | promoter Promoter name: CMV | promoter Promoter name: CMV |
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| concentration 20 ng/μL in TE buffer; DNA (1μg of plasmid DNA) | concentration 20 ng/μL in TE buffer; DNA (1μg of plasmid DNA) | concentration 20 ng/μL in TE buffer; DNA (1μg of plasmid DNA) | concentration 20 ng/μL in TE buffer; DNA (1μg of plasmid DNA) |
| selection kanamycin | selection kanamycin | selection - | selection kanamycin |
General description
Application
- Creation of gene knockouts in multiple cell lines
- Complete knockout of genes not amenable to RNAi
- Creation of knock-in cell lines with promoters, fusion tags or reporters integrated into endogenous genes
Biochem/physiol Actions
Physical form
Preparation Note
Other Notes
Please note, product does not contain guideRNA sequence. This must be purchased separately through the Custom CRISPR product tab.
Typical transfection concentrations used in literature are in the ranges of >= 1.0 ug/uL and <= 5 uL of Cas9 plasmid combined with >= 1.0 ug/uL and <= 5 uL of U6-gRNA plasmids. (All dosages above assume 0.5 to 1 million cells nucleofected)
Legal Information
Lagerklasse
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
nwg
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Protokolle
Learn about CRISPR Cas9, what it is and how it works. CRISPR is a new, affordable genome editing tool enabling access to genome editing for all.
Verwandter Inhalt
Sigma-Aldrich® Advanced Genomics est le premier fournisseur de technologies d'édition et d'extinction géniques, dont CRISPR, Cas9, les ARN guides synthétiques (ARNsg) et les nucléases à doigts de zinc (ZFN pour "Zinc Finger Nucleases").
Sigma-Aldrich® Advanced Genomics è il fornitore leader di tecnologie di editing e silenziamento genico tra cui CRISPR, Cas9, RNA guida sintetici (sgRNA) e nucleasi a dita di zinco (ZFN).
Sigma-Aldrich® Advanced Genomics is the leading provider of gene editing and silencing technologies including CRISPR, Cas9, synthetic guide RNA (sgRNA), and Zinc Finger Nuclease (ZFN).
Sigma-Aldrich® Advanced Genomics ist der führende Anbieter von Technologien für die Geneditierung und Genstummschaltung wie CRISPR, Cas9, Single Guide-RNA (sgRNA) und Zinkfingernuklease (ZFN).
Global Trade Item Number
| SKU | GTIN |
|---|---|
| CAS9P-1EA | 04061833531556 |
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