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Anti-MAP1LC3B Antibody

Name: Anti-MAP1LC3B Antibody
Brand: SIGMA
Price: 576 USD
Availability: InStock

rabbit polyclonal

Enhanced Validation

Synonym(s):

LC3B Antibody - Anti-LC3B antibody produced in rabbit, Lc3 Antibody Sigma, Lc3B Antibody, Anti-Autophagy-related protein LC3 B, Anti-Autophagy-related ubiquitin-like modifier LC3 B, Anti-MAP1 light chain 3-like protein 2, Anti-MAP1A/1B light chain 3 B, Anti-MAP1A/MAP1B LC3 B, Anti-Microtubule-associated proteins 1A/B light chain 3B precursor

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Size/SKU	Availability	Price
100 μL	Available to ship TODAYfromMILWAUKEE	$576.00
200 μL	Available to ship TODAYfromMILWAUKEE	$762.00

About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD09265362

Conjugate:

unconjugated

Clone:

polyclonal

Application:

IF, WB

Citations:

1586

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Properties

Product Name

Anti-LC3B antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 16-18 kDa

species reactivity

mouse, human, rat

enhanced validation

functional assay
Learn more about Antibody Enhanced Validation

concentration

~1 mg/mL

technique(s)

indirect immunofluorescence: 5-10 μg/mL using human HeLa cells, western blot: 1-2 μg/mL using whole extracts of rat and mouse brain

UniProt accession no.

Q9GZQ8

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MAP1LC3B(81631)
mouse ... Map1lc3b(67443)
rat ... Map1lc3b(64862)

Description

General description

Microtubule-associated protein 1 light chain 3 (MAPLC3/LC3) was first identified in rat as a protein that co-purifies with microtubule-associated protein 1A and 1B from rat brain. LC3 exists in cells in two forms. One is cytoplasmic, LC3-I (18 kDa) and the other, LC3-II (16 kDa) is associated with the autophagosome membrane. LC3-I is modified to a membrane-bound form, LC3-II (a LC3-phospholipid conjugate), by mammalian autophagy related protein 7 (Atg7) and Atg3, which are E1- and E2-like enzymes, respectively. Three human orthologs of the rat LC3, named MAP1LC3A/LC3A, MAP1LC3B/LC3B, and MAP1LC3C/ LC3C, were identified. The human proteins exhibit two forms representing the cytosolic (type I) and the membrane associated (type II) forms. The three human isoforms show different expression patterns in human tissues.

The gene MAP1LC3B (microtubule associated protein 1 light chain 3 β) is a homolog of ATG8 gene, an autophagy-related gene in yeast. The encoded protein is called pro-MAP1LC3B, a pro-form that is cleaved by ATG4 to produce a cytosolic form called MAP1LC3B-I.

Immunogen

synthetic peptide corresponding to amino acids 2-15 of human LC3B, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence differs by one amino acid in rat and mouse.

Application

Anti-LC3B antibody produced in rabbit has been used in western blotting and immunohistochemistry.

Biochem/physiol Actions

By immunoblotting, Anti-LC3B antibody recognizes human, rat, and mouse LC3B-I and LC3B-II (~18 kDa and ~16 kDa, respectively).

MAP1LC3B-I (microtubule associated protein 1 light chain 3 β-I) conjugates with MAP1LC3B-II before being inserted into both inner and outer membranes of the growing autophagic vesicle during autophagy. The cellular distribution and processing of MAP1LC3B facilitates monitoring of autophagy. Cells that lack MAP1LC3B are found to be defective in the formation of autophagic vesicles, with altered phenotypes associated with the role of autophagy in energy homeostasis and removal of protein aggregates. It can serve as an autophagic marker.

Microtubule-associated protein 1 light chain 3 (MAPLC3/LC3) expression level correlates with the extent of autophagosome formation. LC3B can be used as an autophagosomal marker.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item	SAB4200361	ZRB100	L8918
Sigma-Aldrich L7543 Anti-LC3B antibody produced in rabbit	Sigma-Aldrich SAB4200361 Anti-LC3B antibody, Mouse monoclonal	Sigma-Aldrich ZRB100 Anti-LC3B Antibody, clone 12K5, ZooMAb^® Rabbit Monoclonal	Sigma-Aldrich L8918 Anti-LC3 antibody produced in rabbit
description ~1 mg/mL, affinity isolated antibody, buffered aqueous solution	description clone LC3B-6, purified from hybridoma cell culture	description recombinant, expressed in HEK 293 cells	description ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
biological source rabbit	biological source mouse	biological source rabbit (recombinant)	biological source rabbit
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate unconjugated
antibody form affinity isolated antibody	antibody form purified immunoglobulin	antibody form purified antibody	antibody form affinity isolated antibody
form buffered aqueous solution	form buffered aqueous solution	form lyophilized	form buffered aqueous solution
shipped in dry ice	shipped in dry ice	shipped in ambient	shipped in dry ice

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Storage Class

10 - Combustible liquids

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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