One potentially suitable anti-laminin antibody for mouse kidney is the L9393 , Anti-Laminin antibody produced in rabbit.
L9393
Anti-Laminin (LAMA1) antibody
Enhanced Validation
rabbit polyclonal
Synonym(s):
Laminin Antibody
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| Size/SKU | Availability | Price |
|---|---|---|
100 μL | Available to ship TODAYfromMILWAUKEE | $560.00 |
0.2 mL | Available to ship TODAYfromMILWAUKEE | $724.00 |
0.5 mL | Available to ship TODAYfromMILWAUKEE | $1,500.00 |
About This Item
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ARR, DB, IHC (p)
Citations:
1671
Product Name
Anti-Laminin antibody produced in rabbit, 0.5 mg/mL, affinity isolated antibody, buffered aqueous solution
biological source
rabbit
Quality Segment
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
animal, human
enhanced validation
independent
Learn more about Antibody Enhanced Validation
concentration
0.5 mg/mL
technique(s)
dot blot: 1:1,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:30 using human and animal tissues, microarray: suitable
UniProt accession no.
application(s)
research pathology
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... LAMA1(284217)
mouse ... Lama1(16772)
General description
Laminin is a ubiquitous non-collagenous connective tissue glycoprotein that is a major constituent of basement membranes. Laminins are extracellular matrix proteins. The LAMA1 (laminin subunit α 1) gene is mapped to human chromosome 18p11. The antibody is isolated from antiserum by immunospecific methods of purification. Antigen specific isolation removes essentially all rabbit serum proteins, including immunoglobulins that do not specifically bind to laminin.
Immunogen
Laminin isolated from the basement membrane of Englebreth Holm-Swarm (EHS) mouse sarcoma.
Application
Anti-Laminin antibody has been used in immunohistochemical staining and immunohistochemistry.
Rabbit Anti-Laminin antibody may be used in immunohistochemistry for marking blood vessel walls in different species, classification of various disease processes involving basement membranes, identification of the origin of human tumors and their classification, and for distinguishing between non-invasive and invasive lesions. The antibody may be used to monitor levels of laminin in biological fluids and for experimental production of basement membrane lesions in vivo.
A working dilution of at least 1:1,000 was determined by a dot blot immunoassay using laminin at 50 ng per dot.
A working dilution of at least 1:25 was determined by indirect immunohistology using formalin-fixed, paraffin-embedded human and animal tissues.
A working dilution of at least 1:1,000 was determined by a dot blot immunoassay using laminin at 50 ng per dot.
A working dilution of at least 1:25 was determined by indirect immunohistology using formalin-fixed, paraffin-embedded human and animal tissues.
Biochem/physiol Actions
LAMA1 (laminin subunit α 1) is involved in several diseases like cancer, infections, inflammatory diseases and autoimmune disorders. Mutations in LAMA1 result in cerebellar dysplasia and cysts with and without retinal dystrophy. LAMA1 is also essential in the assembly of basement membrane and early growth of embryo.
Specificity of the anti-laminin antibodies is determined by indirect immunofluorescent labeling of formalin-fixed, paraffin-embedded human or animal tissue sections, and by dot blot immunoassay. By indirect immunofluorescence the antibody demonstrates specific basement membrane staining of enzymatically unmasked human and animal tissue. In the dot blot immunoassay the rabbit anti-laminin antibody reacts with laminin but not with fibronectin, vitronectin, collagen IV, or chondroitin sulfate types A, B, and C. The affinity isolated antibody to laminin will react with laminin of human, mammal, avian, reptilian, and amphibian sources.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| description 0.5 mg/mL, affinity isolated antibody, buffered aqueous solution | description clone LAM-89, purified from hybridoma cell culture | description clone LAM-89, purified from hybridoma cell culture | description Ab1, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| conjugate unconjugated | conjugate - | conjugate unconjugated | conjugate unconjugated |
| antibody form affinity isolated antibody | antibody form purified from hybridoma cell culture | antibody form purified from hybridoma cell culture | antibody form affinity isolated antibody |
| biological source rabbit | biological source mouse | biological source mouse | biological source rabbit |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in wet ice |
| technique(s) dot blot: 1:1,000, microarray: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:30 using human and animal tissues | technique(s) ELISA: suitable, electron microscopy: suitable, immunoblotting: 0.03-0.06 μg/mL using Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane, immunofluorescence: suitable, immunohistochemistry: 20-40 μg/mL using pronase-retrieved formalin-fixed, paraffin-embedded human tongue sections. | technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using enzyme-treated human tissue sections, indirect ELISA: suitable, western blot: suitable | technique(s) immunoblotting: 0.04-0.4 μg/mL, immunohistochemistry: 1:500-1:1000 |
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Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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What are the best suggestions for an anti-laminin antibody suitable for mouse kidney?
1 answer-
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What is the isotope of the anti-laminin L9393 antibody so that the proper secondary isotope can be used?
1 answer-
The product L9393, an anti-laminin antibody produced in rabbit, is a polyclonal antibody and does not have a single isotype. It is rabbit IgG.
Helpful?
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What applications is Anti-Laminin antibody, Product L9393, suitable for?
1 answer-
Rabbit Anti-Laminin may be used in immunohistochemistry, immunofluorescence, and dot blot analysis. This information is located on the product datasheet available on our website.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the species reactivity of Anti-Laminin antibody, Product L9393?
1 answer-
This affinity-isolated antibody to laminin will react with laminin of human, mammal, avian, reptilian, and amphibian sources. This information is located on the product data sheet.
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Can Anti-Laminin antibody, Product L9393, be used in Western blot analysis?
1 answer-
The following reference uses this antibody for Western blot at 1:1000. DiProspero, N.A., et al., Inflammatory Cytokines Interact to Modulate Extracellular Matrix and Astrocytic Support of Neurite Outgrowth. Exp. Neurol., 148, 628-639 (1997).
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What is the protein concentration of Anti-Laminin antibody, Product L9393?
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The protein concentration is Lot/Batch dependent and is located on the Certificate of Analysis.
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