The product is provided as a 0.01% sterile filtered solution in water. When coating slides with this solution, it is recommended to incubate for 5 minutes and allow the slides to dry at room temperature.
P4707
Poly-ʟ-Lysine Hydrobromide
synthetic, liquid, 0.01%, suitable for cell culture
Poly-L-lysine solution
Synonym(s):
PLL Solution
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 50 mL | Check Cart for Availability | $140.00 |
About This Item
Biological source:
synthetic
Form:
liquid
Technique(s):
cell culture | mammalian: suitable
Concentration:
0.01%
Mol wt:
70—150 kDa
Product Name
Poly-L-lysine solution, 0.01%, sterile-filtered, BioReagent, suitable for cell culture
biological source
synthetic
Quality Level
sterility
sterile-filtered
product line
BioReagent
form
liquid
mol wt
70—150 kDa
packaging
pkg of 50 mL
concentration
0.01%
technique(s)
cell culture | mammalian: suitable
surface coverage
4 μg/cm2
impurities
endotoxin, tested
solubility
water: soluble
shipped in
ambient
storage temp.
2-8°C
SMILES string
N([C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O)C(=O)[C@@H](N)CCCCN
InChI
1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1
InChI key
WBSCNDJQPKSPII-KKUMJFAQSA-N
General description
Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.
Application
Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule.
- Poly-L-lysine coated glass coverslips have been used for mounting samples for scanning electron microscopy.
- Poly-L-lysine coated wells were used for trapping of PC-12 cells on electrodes.
- Poly-L-lysine-coated borosilicate glass slides were used for the seeding of viral vector-transduced cells.
Biochem/physiol Actions
Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.
Preparation Note
This product is supplied as a 0.01% sterile filtered solution in water. Coating slides with this solution should be followed with a 5 minute incubation and drying the slides at room temperature. Coated slides will be stable for one year if protected from dust.
Other Notes
Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.
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This Item | |||
|---|---|---|---|
| sterility sterile-filtered | sterility γ-irradiated | sterility γ-irradiated | sterility - |
| biological source synthetic | biological source synthetic (organic) | biological source synthetic (organic) | biological source synthetic |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - |
| concentration 0.01% | concentration - | concentration - | concentration 0.1 % (w/v) in H2O |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| mol wt 70—150 kDa | mol wt 70,000-150,000 | mol wt 30,000-70,000 | mol wt 150,000-300,000 |
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Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Protocols
Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.
Articles
Poly-Lysine enhances cell binding with positively-charged surface ions, optimizing electrostatic interaction on culture surfaces for increased cell attachment.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| P4707-50ML | 04061835569151 |
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When coating coverslips with item P4707, is it necessary to wash the reagent off after 5 minutes, or is there a different recommended procedure?
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Is the product P4707 suitable for coating glassware for cell culture? Also, what is the difference between Poly L and Poly D lysine?
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Yes, the product P4707 is suitable for coating glassware for cell culture. The difference between Poly L and Poly D lysine lies in their stereoisomeric properties. Poly-L-Lysine is a charge enhancer and can be used for coating many surfaces, including tissue culture plasticware and glassware. However, certain cells can digest poly-L-lysine, in which case the poly-D-lysine, such as P7405, should be used. This product can also be used to coat tissue culture plasticware and glassware.
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How many times can it be reused?
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The number of times that this product may be re-applied has not been determined. This would be dependent on several factors, such as surface area, material type, surface pretreatment, and incubation period. Please see the link below to review the product datasheet for additional information:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/244/731/p4707pis.pdfHelpful?
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What is the molecular weight of product P4707?
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Poly-L-lysine solution is 70-150 kDa molecular weight.
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What is the protocol for using Product P4707, Poly-L-Lysine solution, to coat slides?
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For coating slides with poly-L-lysine, in general, it is recommended to use a 0.01% (w/v) solution. After a 5 minute incubation, the excess solution is removed and the slides are dried at room temperature or in an oven at gentle heat.
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Should I use Poly-L-Lysine or Poly-D-Lysine?
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Certain cells secrete proteases, which can digest poly-L-lysine. For those cell types, poly-D-lysine should be used.
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What molecular weight Poly-L-Lysine product should I choose?
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The lower molecular weight poly-L-lysine (30-70 kDa) is easier to use because it is less viscous in solution, but the higher molecular weight poly-L-lysine (>300 kDa) provides more attachment sites per molecule. The mid-range product has a molecular weight range of 70-150 kDa.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How can I sterilize slides coated with Product P4707, Poly-L-Lysine solution?
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Poly-L-Lysine coated slides can be exposed to UV light overnight for sterilization.
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What is the protocol for using Product P4707, Poly-L-Lysine solution, to coat tissue culture plasticware?
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A solution of 0.1 mg/mL is prepared; 0.5 mL of that solution is aseptically transferred to a non-tissue culture treated 25 square cm flask. The flask is gently rocked to evenly coat the surface. After five minutes, the excess solution is removed and the surface is thoroughly rinsed with sterile water and allowed to dry for several hours.
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